Mycobacterium phage EmpTee
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Detailed Information for Phage EmpTee
Discovery Information
Isolation HostMycobacterium smegmatis mc²155
Former namesEmpTee
Found ByMargaret Tish
Year Found2013
Location FoundPittsburgh, PA USA
Finding InstitutionUniversity of Pittsburgh
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?Yes
Isolation TemperatureNot entered
GPS Coordinates40.44 N, 79.96 W Map
Discovery NotesThe soil was collected from the edge of the lawn on the Soldiers and Sailors memorial. It was collected from the West side behind the parking garage entrance.
Naming NotesThe phage is named EmpTee after my initial MT, which when said sound like empty.
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedNov 13, 2013
Sequencing FacilityPittsburgh Bacteriophage Institute
Shotgun Sequencing MethodIllumina Sequencing
Approximate Shotgun Coverage7252
Genome length (bp)68428
Character of genome endsCircularly Permuted
GC Content66.5%
Fasta file available?Yes: Download fasta file
Characterization
ClusterB
SubclusterB1
Cluster Life CycleLytic
Lysogeny NotesNo Lysogens created
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DaddyDaniels (B1)

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Daka (B1)

Dati (B1)

DelRivs (B1)

Derpp (B1)

Dice (B1)

Dingo (B1)

Dione (B1)

DirtJuice (B1)

Doddsville (B1)

DoesntMatter (B1)

DonSanchon (B1)

DuchessDung (B1)

Duggie (B1)

Durga (B1)

Emiris (B1)

EmpTee (B1)

Eremos (B1)

Etaye (B1)

Eugenia (B1)

Fang (B1)

FluffyNinja (B1)

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Lolalove (B4)

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Mithril (B4)

Mudslide (B4)

Nanao (B4)

Nigel (B4)

Poster (B4)

Prince (B4)

QueenPie (B4)

RawrgerThat (B4)

Stinger (B4)

Vincenzo (B4)

VioletZ (B4)

Waleliano (B4)

Xincheng (B4)

Zemanar (B4)

Acadian (B5)

Baee (B5)

DigitDog (B5)

Donny (B5)

EastView9101 (B5)

KarasumaRum (B5)

Mysterious (B5)

Nangang (B5)

Nehemiah (B5)

Phelemich (B5)

Reprobate (B5)

Rich (B5)

Serendipitous (B5)

Shenyu (B5)

SirJeffery (B5)

StinkyVeggy (B5)

Suigeneris (B5)

39HC (B6)

40BC (B6)

Hosp (B6)

Jolie1 (B6)

KayaCho (B6)

Saguaro (B7)

Thonko (B8)

CRB2 (B9)

Funsized (B9)

Knocker (B9)

PenguinLover67 (B9)

Quesadilla (B9)

Rinkes (B9)

Annotating InstitutionUnknown or unassigned
Annotation StatusIn GenBank
Plaque NotesOn a plate, it appears that there are multiple phage because there are large plaques and small plaques, but each plaque has a cloud around it, and it is impossible to isolate either phage.
MorphotypeSiphoviridae
Number of Genes101
Number of tRNAs0
Number of tmRNAs0
Has been Phamerated?Yes
Gene List

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EmpTee_1
(adenylate kinase)

EmpTee_2
(terminase)

EmpTee_3

EmpTee_4

EmpTee_5

EmpTee_6
(RuvC-like resolvase)

EmpTee_7

EmpTee_8
(portal protein)

EmpTee_9
(capsid maturation protease and MuF-like fusion protein)

EmpTee_10
(HNH endonuclease)

EmpTee_11

EmpTee_12
(major capsid hexamer protein)

EmpTee_13
(major capsid pentamer protein)

EmpTee_14

EmpTee_15

EmpTee_16

EmpTee_17

EmpTee_18
(major tail protein)

EmpTee_19

EmpTee_20
(queuine tRNA-ribosyltransferase)

EmpTee_21

EmpTee_22
(head-to-tail adaptor)

EmpTee_23

EmpTee_24

EmpTee_25
(tail assembly chaperone)

EmpTee_26

EmpTee_27

EmpTee_28
(tape measure protein)

EmpTee_29
(minor tail protein)

EmpTee_30
(minor tail protein)

EmpTee_31
(minor tail protein)

EmpTee_32
(minor tail protein)

EmpTee_33
(minor tail protein)

EmpTee_34

EmpTee_35

EmpTee_36

EmpTee_37

EmpTee_38

EmpTee_39

EmpTee_40

EmpTee_41

EmpTee_42

EmpTee_43

EmpTee_44

EmpTee_45
(helix-turn-helix DNA binding domain protein)

EmpTee_46

EmpTee_47

EmpTee_48
(lysin A)

EmpTee_49
(lysin B)

EmpTee_50

EmpTee_51

EmpTee_52
(DNA helicase)

EmpTee_53

EmpTee_54

EmpTee_55

EmpTee_56

EmpTee_57
(DNA primase/helicase)

EmpTee_58

EmpTee_59

EmpTee_60
(DNA polymerase I)

EmpTee_61

EmpTee_62

EmpTee_63

EmpTee_64

EmpTee_65

EmpTee_66

EmpTee_67

EmpTee_68

EmpTee_69

EmpTee_70

EmpTee_71

EmpTee_72

EmpTee_73

EmpTee_74

EmpTee_75

EmpTee_76

EmpTee_77

EmpTee_78

EmpTee_79

EmpTee_80

EmpTee_81

EmpTee_82

EmpTee_83
(HNH endonuclease)

EmpTee_84

EmpTee_85

EmpTee_86

EmpTee_87

EmpTee_88

EmpTee_89

EmpTee_90

EmpTee_91

EmpTee_92

EmpTee_93

EmpTee_94

EmpTee_95

EmpTee_96

EmpTee_97

EmpTee_98

EmpTee_99

EmpTee_100

EmpTee_101

Publication Info
Uploaded to GenBank?Yes
GenBank AccessionKJ567044
Refseq NumberNone yet
Published in PaperPope, Bowman, Russell, et al 2015, eLIFE: Whole genome comparison of a large collection of mycobacteriophages reveals a continuum of phage genetic diversity
Archiving Info
Archiving status Archived
Pitt Freezer Box# 1
Pitt Freezer Box Grid# A8
Available Files
Plaque PictureDownload
Restriction Digest PictureDownload
EM PictureDownload