| Detailed Information for Phage Fozzie |
| Discovery Information |
| Isolation Host | Mycobacterium smegmatis mc²155 |
| Former names | Mahnamahna |
| Duplicates of this Phage | Qwerbl |
| Found By | Elena Marie Sable |
| Year Found | 2022 |
| Location Found | Roanoke, VA United States |
| Finding Institution | Virginia Western Community College |
| Program | Unavailable |
| From enriched soil sample? | Yes |
| Isolation Temperature | 37°C |
| GPS Coordinates | 37.254749 N, 79.95962 W Map |
| Discovery Notes | One of our lab members collected the soil from outside of Chipotle. The soil was pretty dry and very light in weight. |
| Naming Notes | We chose this name because we always sing muppet songs during lab. |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Jan 5, 2023 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina |
| Sequencer Used | Illumina MiSeq |
| Read Type | Single-end reads |
| Read Length | 150 bp |
| Approximate Shotgun Coverage | 534 |
| Genome length (bp) | 68992 |
| Character of genome ends | Circularly Permuted |
| GC Content | 66.4% |
| Sequencing Notes | When sequenced, this phage's DNA sample was found to be contaminated with phage DrFeelGood (~0.62 times the coverage of the main phage). |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | B |
| Subcluster | B1 |
| Cluster Life Cycle | Lytic |
| Other Cluster Members |
|
| Annotating Institution | Virginia Western Community College |
| Annotation Status | In GenBank |
| Plaque Notes | It is 0.4 cm. Is is very easy to see. |
| Morphotype | Siphoviridae |
| Number of Genes | 98 |
| Number of tRNAs | 0 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Submitted Annotation in DNA Master Format | Download |
| Submitted Minimal DNA Master File | Download |
| Author List | Download |
| Annotation Cover Sheet | Download |
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | OR434028 |
| Refseq Number | None yet |
| Archiving Info |
| Archiving status |
Archived |
| SEA Lysate Titer |
5.475 x 10⁹ |
| Pitt Freezer Box# |
153 |
| Pitt Freezer Box Grid# |
H10 |
| Available Files |
| Plaque Picture | Download |
| GenBank File for Phamerator | Download |
