| Detailed Information for Phage Halfpint |
| Discovery Information |
| Isolation Host | Mycobacterium smegmatis mc²155 |
| Former names | N/A |
| Found By | Madeline Tinskey |
| Year Found | 2023 |
| Location Found | Commerce Township, MI United States of America |
| Finding Institution | Hope College |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | No |
| Isolation Temperature | 34°C |
| GPS Coordinates | 42.58 N, 83.49 W Map |
| Discovery Notes | This phage was found in an enriched water sample obtained from North Commerce Lake, Michigan. The sample site was a freshwater lake with seaweed, kelp, and sand. The lake levels and humidity in the air were particularly high on the day this sample was collected as there were recent storms. |
| Naming Notes | My favorite books growing up were the "Little House on the Prairie" series. The author, Laura Ingalls Wilder, writes often of the endearing nickname her father gave her: "half-pint". I wanted to pay homage to one of the most cherished stories of my youth. Additionally, half-pint indicates a very small person just as my phage plaques are small. |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Jan 2, 2024 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina |
| Approximate Shotgun Coverage | 1528 |
| Genome length (bp) | 68449 |
| Character of genome ends | Circularly Permuted |
| GC Content | 67.5% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | B |
| Subcluster | B3 |
| Cluster Life Cycle | Lytic |
| Lysogeny Notes | Plaques are generally very clear as opposed to turbid, indicating the the phage is probably lytic. |
| Other Cluster Members |
|
| Annotating Institution | Hope College |
| Annotation Status | In GenBank |
| Plaque Notes | Plaques are very small (less than 0.5 mm) in size, and clear. This indicates that the phage is probably lytic. The plaques are round in shape and the size of pinpricks. |
| Morphotype | Siphoviridae |
| Number of Genes | 102 |
| Number of tRNAs | 0 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Submitted Annotation in DNA Master Format | Download |
| Submitted Minimal DNA Master File | Download |
| Author List | Download |
| Annotation Cover Sheet | Download |
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | PQ184808 |
| Refseq Number | None yet |
| Archiving Info |
| Archiving status |
Archived |
| Pitt Freezer Box# |
182 |
| Pitt Freezer Box Grid# |
F7 |
| Available Files |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| EM Picture | Download |
| GenBank File for Phamerator | Download |
