| Detailed Information for Phage Hamish |
| Discovery Information |
| Isolation Host | Mycobacterium smegmatis mc²155 |
| Former names | NicolasPhage, Bellamy |
| Found By | Hallie Smith |
| Year Found | 2016 |
| Location Found | Rock Hill, SC USA |
| Finding Institution | Winthrop University |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Unknown |
| Isolation Temperature | 37°C |
| GPS Coordinates | 34.9371 N, 81.032 W Map |
| Discovery Notes | Dry soil approximately 1 inch under top layer of soil. Obtained at 10:30 pm on 9/7/16 at Hardin Family Garden at Winthrop University. 29.44 °C |
| Naming Notes | Named after John Hamish Watson from BBC Sherlock. |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Feb 23, 2017 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina |
| Sequencer Used | Illumina MiSeq |
| Read Type | Single-end reads |
| Read Length | 150 bp |
| Approximate Shotgun Coverage | 824 |
| Genome length (bp) | 68584 |
| Character of genome ends | Circularly Permuted |
| GC Content | 66.5% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | B |
| Subcluster | B1 |
| Cluster Life Cycle | Lytic |
| Other Cluster Members |
|
| Annotating Institution | Winthrop University |
| Annotation Status | In GenBank |
| Plaque Notes | Average head diameter: 70.382 nm
Average tail length: 345.908 nm |
| Morphotype | Siphoviridae |
| Number of Genes | 99 |
| Number of tRNAs | 0 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Submitted Annotation in DNA Master Format | Download |
| Submitted Minimal DNA Master File | Download |
| Author List | Download |
| Annotation Cover Sheet | Download |
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | MH825702 |
| Refseq Number | None yet |
| Archiving Info |
| Archiving status |
Archived |
| SEA Lysate Titer |
2.3e10 pfu/ml |
| Date of SEA Lysate Titering |
Sep 22, 2016 |
| Pitt Freezer Box# |
43 |
| Pitt Freezer Box Grid# |
B3 |
| Available Files |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| EM Picture | Download |
| GenBank File for Phamerator | Download |
