| Detailed Information for Phage Hashim76 |
| Discovery Information |
| Isolation Host | Mycobacterium smegmatis mc²155 |
| Found By | Hams Kamil |
| Year Found | 2022 |
| Location Found | Hanahan, SC United States |
| Finding Institution | College of Charleston |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | 37°C |
| GPS Coordinates | 32.95276 N, 80.03173 W Map |
| Discovery Notes | Soil sample was collected in Hanahan, SC at 10:07AM on 9/23/2022 from a tire track in mud near a pond. It was 66 degrees Fahrenheit outside with slight wind. The soil was wet and clumpy and dark brown in color. |
| Naming Notes | This phage was named after my mother. Her last name is Hashim and she was born in 1976. |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Feb 20, 2023 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina |
| Sequencer Used | Illumina MiSeq |
| Read Type | Single-end reads |
| Read Length | 150 bp |
| Approximate Shotgun Coverage | 955 |
| Genome length (bp) | 70230 |
| Character of genome ends | Circularly Permuted |
| GC Content | 70.1% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | B |
| Subcluster | B13 |
| Cluster Life Cycle | Lytic |
| Other Cluster Members |
|
| Annotating Institution | College of Charleston |
| Annotation Status | In GenBank |
| Plaque Notes | Plaque that was later picked was circular in shape and clear. |
| Morphotype | Siphoviridae |
| Number of Genes | 101 |
| Number of tRNAs | 0 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Submitted Minimal DNA Master File | Download |
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | PQ844485 |
| Refseq Number | None yet |
| Archiving Info |
| Archiving status |
Archived |
| Pitt Freezer Box# |
163 |
| Pitt Freezer Box Grid# |
H6 |
| Available Files |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| EM Picture | Download |
| GenBank File for Phamerator | Download |
