| Detailed Information for Phage HighStump |
| Discovery Information |
| Isolation Host | Mycobacterium smegmatis mc²155 |
| Found By | Andrew Schaff |
| Year Found | 2015 |
| Location Found | Oxford, OH USA |
| Finding Institution | Miami University |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | Not entered |
| GPS Coordinates | 39.510534 N, 84.739384 W Map |
| Discovery Notes | Found at the base of a stump. An active construction site was approximately 10 feet away. |
| Naming Notes | Named because the soil sample was taken from the base of a stump off of High Street. |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Jan 14, 2016 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina |
| Sequencer Used | Illumina MiSeq |
| Read Type | Single-end reads |
| Read Length | 150 bp |
| Approximate Shotgun Coverage | 1644 |
| Genome length (bp) | 68821 |
| Character of genome ends | Circularly Permuted |
| GC Content | 66.5% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | B |
| Subcluster | B1 |
| Cluster Life Cycle | Lytic |
| Other Cluster Members |
|
| Annotating Institution | Unknown or unassigned |
| Annotation Status | In GenBank |
| Plaque Notes | lytic, circular plaques. Bullseye pattern and approximately 4.7 mm across. |
| Morphotype | Siphoviridae |
| Number of Genes | 104 |
| Number of tRNAs | 0 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Submitted Annotation in DNA Master Format | Download |
| Submitted Minimal DNA Master File | Download |
| Author List | Download |
| Annotation Cover Sheet | Download |
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | MG757158 |
| Refseq Number | None yet |
| Archiving Info |
| Archiving status |
Archived |
| SEA Lysate Titer |
9.4x10^10 |
| Date of SEA Lysate Titering |
Oct 27, 2015 |
| Pitt Freezer Box# |
24 |
| Pitt Freezer Box Grid# |
F2 |
| Available Files |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| EM Picture | Download |
| Final DNAMaster File | Download |
| GenBank File for Phamerator | Download |
