| Detailed Information for Phage Holeinone | 
	
	
		| Discovery Information | 
		| Isolation Host | Mycobacterium smegmatis mc²155 | 
		
		
		
		
		| Found By | Oriana Jerez | 
		| Year Found | 2016 | 
		| Location Found | Charlotte, NC  USA | 
		| Finding Institution | Queens University of Charlotte | 
		| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science | 
		| From enriched soil sample? | Yes | 
		| Isolation Temperature | Not entered | 
		| GPS Coordinates | 35.1789 N, 80.8341 W  Map | 
		| Discovery Notes | Found on a golf coarse under a tree, near the 18th green. | 
		| Naming Notes | Since the bacteriophage was found on a golf coarse, I decided to name it "Holeinone" since getting a hole-in-one in golf is really hard to get.  | 
		
                
		| Sequencing Information | 
		| Sequencing Complete? | Yes | 
		| Date Sequencing Completed | Jan 3, 2017 | 
		| Sequencing Facility | Pittsburgh Bacteriophage Institute | 
		| Shotgun Sequencing Method | Illumina | 
		| Sequencer Used | Illumina MiSeq | 
		| Read Type | Single-end reads | 
		| Read Length | 150 bp | 
		| Approximate Shotgun Coverage | 2992 | 
		
		| Genome length (bp) | 67044 | 
		| Character of genome ends | Circularly Permuted | 
		
		
		
		
		| GC Content | 68.9% | 
		
		| Fasta file available? | Yes: Download fasta file | 
		| Characterization | 
		| Cluster | B | 
		| Subcluster | B2 | 
                | Cluster Life Cycle | Lytic | 
		
		| Other Cluster Members | 
                
		 | 
		| Annotating Institution | Unknown or unassigned | 
                | Annotation Status | In GenBank | 
		| Plaque Notes | Really tiny plaques. | 
		| Morphotype | Siphoviridae | 
		| Number of Genes | 91 | 
		| Number of tRNAs | 0 | 
		| Number of tmRNAs | 0 | 
		| Has been Phamerated? | Yes | 
		| Gene List | 
			
		 | 
		| Submitted Annotation in DNA Master Format | Download | 
		| Submitted Minimal DNA Master File | Download | 
		| Author List | Download | 
		| Annotation Cover Sheet | Download | 
		| Publication Info | 
		| Uploaded to GenBank? | Yes | 
		| GenBank Accession | MG812490 | 
		| Refseq Number | None yet | 
		
        
        | Archiving Info | 
        
        
          | Archiving status | 
          Archived | 
        
        
        
        
        
        
        | Pitt Freezer Box# | 
        45 | 
        
        
        
        
        | Pitt Freezer Box Grid# | 
        B9 | 
        
        
        
		
		| Available Files | 
		| Plaque Picture | Download | 
		
		
		| EM Picture | Download | 
		
		| Final DNAMaster File | Download | 
		| GenBank File for Phamerator | Download |