| Detailed Information for Phage Inchworm |
| Discovery Information |
| Isolation Host | Mycobacterium smegmatis mc²155 |
| Found By | Cecilia Petruconis |
| Year Found | 2019 |
| Location Found | Philadelphia, PA United States |
| Finding Institution | Saint Joseph's University |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | No |
| Isolation Temperature | 36°C |
| GPS Coordinates | 39.9941 N, 75.2379 W Map |
| Discovery Notes | Inchworm was found under the large pine tree at the top of the staircase next to Bellarmine Hall on the Saint Joseph's University Campus. Inchworm came from Direct Isolation, followed by 2 more rounds of purification. The Titer was 3.8 x 10^10 pfu/mL. The DNA concentration was 111.6 μg/mL. The average capsid size was 58.3 nm in diameter, and the average tail was 312.5 nm in length. |
| Naming Notes | When I collected this sample, I dug up an inchworm and put it into the sample collection by accident. I removed the inchworm before returning to the lab, but the name stuck. |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Feb 5, 2020 |
| Sequencing Facility | NC State Genomic Sciences Laboratory |
| Shotgun Sequencing Method | Illumina |
| Approximate Shotgun Coverage | 2173 |
| Genome length (bp) | 68929 |
| Character of genome ends | Circularly Permuted |
| GC Content | 66.3% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | B |
| Subcluster | B1 |
| Cluster Life Cycle | Lytic |
| Other Cluster Members |
|
| Annotating Institution | Saint Joseph's University |
| Annotation Status | In GenBank |
| Plaque Notes | The plaques for all rounds of purification were 3 mm diameter. They were clear plaques, reflecting a lytic phage. They were symmetrical and had clear edges and were consistent throughout isolation. |
| Morphotype | Siphoviridae |
| Number of Genes | 103 |
| Number of tRNAs | 0 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Submitted Annotation in DNA Master Format | Download |
| Submitted Minimal DNA Master File | Download |
| Author List | Download |
| Annotation Cover Sheet | Download |
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | MT889369 |
| Refseq Number | None yet |
| Archiving Info |
| Archiving status |
Archived |
| SEA Lysate Titer |
2.1 x 10^9 pfu/mL |
| Pitt Freezer Box# |
101 |
| Pitt Freezer Box Grid# |
G5 |
| Available Files |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| EM Picture | Download |
| GenBank File for Phamerator | Download |
