| Detailed Information for Phage ItsyBitsy1 |
| Discovery Information |
| Isolation Host | Mycobacterium smegmatis mc²155 |
| Former names | DangGina |
| Found By | Katie Mustin, Benita Platas, Stacie Williams |
| Year Found | 2016 |
| Location Found | Wylie, TX USA |
| Finding Institution | Collin College |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | No |
| Isolation Temperature | Not entered |
| GPS Coordinates | 32.978679 N, 96.52515 W Map |
| Discovery Notes | The phage was found in a residential neighborhood in a flower bed near a home.
When obtained there was a large and thick layer of mulch at the top of the soil. The soil was loose and dry the temperature was 76 degrees F and a cloudy morning (7:52 a.m.) |
| Naming Notes | Named after a saying. |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Jan 2, 2017 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina Sequencing |
| Approximate Shotgun Coverage | 3332 |
| Genome length (bp) | 67570 |
| Character of genome ends | Circularly Permuted |
| GC Content | 68.9% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | B |
| Subcluster | B2 |
| Cluster Life Cycle | Lytic |
| Other Cluster Members |
|
| Annotating Institution | Unknown or unassigned |
| Annotation Status | In GenBank |
| Plaque Notes | The size of the plaques are very tiny, they ranged between 0.5 mm-1.5 mm. They also grew clear and in a circular shape. |
| Morphotype | Siphoviridae |
| Number of Genes | 89 |
| Number of tRNAs | 0 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Submitted Annotation in DNA Master Format | Download |
| Submitted Minimal DNA Master File | Download |
| Author List | Download |
| Annotation Cover Sheet | Download |
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | MG812491 |
| Refseq Number | None yet |
| Archiving Info |
| Archiving status |
Archived |
| SEA Lysate Titer |
2.4x10^10 |
| Pitt Freezer Box# |
32 |
| Pitt Freezer Box Grid# |
G1 |
| Available Files |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| EM Picture | Download |
| Final DNAMaster File | Download |
| GenBank File for Phamerator | Download |
