| Detailed Information for Phage JAMaL |
| Discovery Information |
| Isolation Host | Mycobacterium smegmatis mc²155 |
| Found By | A. Bailey, M. Nitzahn, J. Tran, and L. Tran |
| Year Found | 2012 |
| Location Found | Los Angeles, CA USA |
| Finding Institution | University of California, Los Angeles |
| Program | Unavailable |
| From enriched soil sample? | Yes |
| Isolation Temperature | Not entered |
| GPS Coordinates | 34.4 N, 118.3 W Map |
| Discovery Notes | Isolated in MIMG 103AL (Spring 2012)and annotated in MIMG 103BL (Fall 2012) by Team Carpe My Phage: Alanna Bailey, Matt Nitzahn, Jessica Tran, and Long Tran
Instructor: Erin Sanders
TA (AL): Jovo Vijanderan
TA (BL): Will Villella
Curation of genome annotation results in winter 2013 by 199 students: Tiffany Chow |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Apr 22, 2013 |
| Sequencing Facility | The UCLA Genotyping and Sequencing Core |
| Shotgun Sequencing Method | 454 Pyrosequencing |
| Approximate Shotgun Coverage | 39 |
| Genome length (bp) | 70841 |
| Character of genome ends | Circularly Permuted |
| GC Content | 68.8% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | B |
| Subcluster | B4 |
| Cluster Life Cycle | Lytic |
| Lysogeny Notes | Phage exhibits a lytic life cycle, as observed by clear, discreet plaques. Annotation also indicates the presence of an endolysin gene as well as the absence of integrases, reaffirming the lytic life cycle. |
| Other Cluster Members |
|
| Annotating Institution | Unknown or unassigned |
| Annotation Status | In GenBank |
| Plaque Notes | Plaques are clear with a diameter of 1.2mm. |
| Morphotype | Siphoviridae |
| Number of Genes | 98 |
| Number of tRNAs | 0 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | KF493881 |
| Refseq Number | NC_023554 |
| Archiving Info |
| Archiving status |
Archived |
| SEA Designator |
2012UCLAjamalLT |
| SEA Lysate Titer |
1.04x10^9 |
| Date of SEA Lysate Titering |
Jun 6, 2012 |
| Pitt Freezer Box# |
3 |
| Pitt Freezer Box Grid# |
E4 |
| Available Files |
| Plaque Picture | Download |
| EM Picture | Download |
| Final DNAMaster File | Download |