| Detailed Information for Phage Kloppinator |
| Discovery Information |
| Isolation Host | Mycobacterium smegmatis mc²155 |
| Found By | Uriel Sanchez |
| Year Found | 2012 |
| Location Found | Baltimore, MD USA |
| Finding Institution | Johns Hopkins University |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Unknown |
| Isolation Temperature | Not entered |
| GPS Coordinates | 39.3325 N, 76.616672 W Map |
| Discovery Notes | Soil sample taken from a heavily vegetated area. |
| Naming Notes | This phage is dedicated to an amazing biology teacher and mentor. |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Feb 6, 2013 |
| Sequencing Facility | Virginia Commonwealth University Nucleic Acids Research Facilities |
| Shotgun Sequencing Method | 454 |
| Sequencer Used | 454 Genome Sequencer FLX |
| Approximate Shotgun Coverage | 110 |
| Sanger Finishing Reactions | 6 |
| Genome length (bp) | 68480 |
| Character of genome ends | Circularly Permuted |
| GC Content | 66.5% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | B |
| Subcluster | B1 |
| Cluster Life Cycle | Lytic |
| Other Cluster Members |
|
| Annotating Institution | Doane University |
| Annotation Status | In GenBank |
| Plaque Notes | Plaques are approximately 4mm in size.
Borders are clearly defined. No haziness or blurred edges.Plaques themselves are almost perfectly circular and are also clear with no blurring. |
| Morphotype | Siphoviridae |
| Number of Genes | 102 |
| Number of tRNAs | 0 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Submitted Annotation in DNA Master Format | Download |
| Submitted Minimal DNA Master File | Download |
| Author List | Download |
| Annotation Cover Sheet | Download |
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | MN585967 |
| Refseq Number | None yet |
| Published in Paper | Pope, Bowman, Russell, et al 2015, eLIFE: Whole genome comparison of a large collection of mycobacteriophages reveals a continuum of phage genetic diversity |
| Archiving Info |
| Archiving status |
Archived |
| SEA Lysate Titer |
3x10^10 pfu/ml! |
| Date of SEA Lysate Titering |
Nov 29, 2012 |
| Pitt Freezer Box# |
13 |
| Pitt Freezer Box Grid# |
C1 |
| Available Files |
| Plaque Picture | Download |
| EM Picture | Download |
| GenBank File for Phamerator | Download |
