| Detailed Information for Phage Kolhan |
| Discovery Information |
| Isolation Host | Mycobacterium smegmatis mc²155 |
| Found By | Allie Kolnok and Chloe Sheehan |
| Year Found | 2024 |
| Location Found | Blacksburg, VA United States |
| Finding Institution | Virginia Tech |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | 37°C |
| GPS Coordinates | 37.2146 N, 80.45363 W Map |
| Discovery Notes | Kolhan was collected from a dirt pile beside a sidewalk and bushes in a suburban setting. It was dry and collected later in the evening at 12C. |
| Naming Notes | Kolhan is named after the beginning of "Kolnok" from partner Allie Kolnok and the end of "Sheehan" from the other partner Chloe Sheehan. |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Jan 15, 2025 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina |
| Sequencer Used | Illumina NextSeq 1000 |
| Read Type | Single-end reads |
| Read Length | 100 bp |
| Approximate Shotgun Coverage | 718 |
| Genome length (bp) | 67387 |
| Character of genome ends | Circularly Permuted |
| GC Content | 69.0% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | B |
| Subcluster | B2 |
| Cluster Life Cycle | Lytic |
| Other Cluster Members |
|
| Annotating Institution | Virginia Tech |
| Annotation Status | Being Annotated (Expected completion by 5/9/2025) |
| Plaque Notes | Plaques were turbid and medium sized.
Morphotype: Siphoviridae |
| Morphotype | Siphoviridae |
| Number of Genes | 93 |
| Number of tRNAs | 0 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Publication Info |
| Uploaded to GenBank? | No |
| GenBank Accession | None yet |
| Refseq Number | None yet |
| Archiving Info |
| Archiving status |
Archived |
| Pitt Freezer Box# |
210 |
| Pitt Freezer Box Grid# |
H6 |
| Available Files |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| EM Picture | Download |
