| Discovery Information |
| Isolation Host | Mycobacterium smegmatis mc²155 |
| Former names | SampleOne |
| Found By | Mitchell Hallman, Zezhong He |
| Year Found | 2011 |
| Location Found | St. Louis, MO USA |
| Finding Institution | Washington University in St. Louis |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | Not entered |
| GPS Coordinates | 38.645145 N, 90.314564 W Map |
| Discovery Notes | collected 9/5/11 at 1:00pm CST
temperature: 80 degrees Fahrenheit
3 centimeters deep
soil was slightly moist
environment: urban, 20 feet from buildings and foot paths, below a tree |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Oct 10, 2013 |
| Sequencing Facility | The McDonnell Genome Institute at Washington University |
| Shotgun Sequencing Method | 454 |
| Sequencer Used | 454 Genome Sequencer FLX |
| Approximate Shotgun Coverage | 204 |
| Sanger Finishing Reactions | 2 |
| Genome length (bp) | 68921 |
| Character of genome ends | Circularly Permuted |
| GC Content | 66.5% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | B |
| Subcluster | B1 |
| Cluster Life Cycle | Lytic |
| Other Cluster Members |
|
| Annotating Institution | University of Pittsburgh |
| Annotation Status | In GenBank |
| Plaque Notes | roughly one millimeter in diameter
rounded, cloudy edges
uniform in size and shape |
| Morphotype | Siphoviridae |
| Number of Genes | 100 |
| Number of tRNAs | 0 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Submitted Minimal DNA Master File | Download |
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | MG925346 |
| Refseq Number | None yet |
| Published in Paper | Pope, Bowman, Russell, et al 2015, eLIFE: Whole genome comparison of a large collection of mycobacteriophages reveals a continuum of phage genetic diversity |
| Archiving Info |
| Archiving status |
Archived |
| SEA Designator |
2011WUSLleelotKH |
| SEA Lysate Titer |
1.25e10 |
| Date of SEA Lysate Titering |
Oct 4, 2011 |
| Pitt Freezer Box# |
31 |
| Pitt Freezer Box Grid# |
25 |
| Available Files |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| EM Picture | Download |
| Final DNAMaster File | Download |
| GenBank File for Phamerator | Download |
