| Detailed Information for Phage Lego3393 |
| Discovery Information |
| Isolation Host | Mycobacterium smegmatis mc²155 |
| Found By | Meghan Muretta |
| Year Found | 2011 |
| Location Found | Philadelphia, PA USA |
| Finding Institution | Saint Joseph's University |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | Not entered |
| GPS Coordinates | 39.992817 N, 75.239675 W Map |
| Discovery Notes | The soil was collected on Saint Joseph's University's campus from a flower bed beside a concrete walkway between Lafarge Resident Hall and Quirk Resident House. The spot where the soil was taken from was about 1 ft away from foot traffic. The sample was taken 4 cm below the surface and was dry. The temperature when the soil was taken was about 26°C. |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Jan 6, 2014 |
| Sequencing Facility | NC State Genomic Sciences Laboratory |
| Shotgun Sequencing Method | Illumina |
| Sequencer Used | Illumina MiSeq |
| Read Type | Single-end reads |
| Read Length | 150 bp |
| Approximate Shotgun Coverage | 14694 |
| Genome length (bp) | 69037 |
| Character of genome ends | Circularly Permuted |
| GC Content | 66.5% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | B |
| Subcluster | B1 |
| Cluster Life Cycle | Lytic |
| Other Cluster Members |
|
| Annotating Institution | Saint Joseph's University |
| Annotation Status | In GenBank |
| Plaque Notes | The plaques were clear. The average size of the plaques was about 1.0 - 2.0 mm in diameter. They usually appeared after 24 hours of incubation. |
| Morphotype | Siphoviridae |
| Number of Genes | 100 |
| Number of tRNAs | 0 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Submitted Annotation in DNA Master Format | Download |
| Submitted Minimal DNA Master File | Download |
| Author List | Download |
| Annotation Cover Sheet | Download |
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | KX620786 |
| Refseq Number | None yet |
| Published in Paper | Pope, Bowman, Russell, et al 2015, eLIFE: Whole genome comparison of a large collection of mycobacteriophages reveals a continuum of phage genetic diversity |
| Archiving Info |
| Archiving status |
Archived |
| SEA Designator |
2011STJOLego3393MM |
| SEA Lysate Titer |
2.3e11 |
| Date of SEA Lysate Titering |
Oct 20, 2011 |
| Pitt Freezer Box# |
27 |
| Pitt Freezer Box Grid# |
47 |
| Available Files |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| EM Picture | Download |
| GenBank File for Phamerator | Download |
