| Detailed Information for Phage LemonSlice |
| Discovery Information |
| Isolation Host | Mycobacterium smegmatis mc²155 |
| Found By | Ying-Chiang Jeffrey Lee and Claire Brownstone |
| Year Found | 2011 |
| Location Found | St. Louis, MO USA |
| Finding Institution | Washington University in St. Louis |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | Not entered |
| GPS Coordinates | 38.643844 N, 90.315294 W Map |
| Discovery Notes | Moisture: Dry
Depth: 3cm
Air Temperature: 66º F
Features: In an urban environment, next to a brick and cement path frequented by foot traffic. Decaying vegetation nearby. In a shadow during parts of the day due to vegetation cover nearby. |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Oct 10, 2013 |
| Sequencing Facility | The McDonnell Genome Institute at Washington University |
| Shotgun Sequencing Method | 454 |
| Sequencer Used | 454 Genome Sequencer FLX |
| Approximate Shotgun Coverage | 180 |
| Sanger Finishing Reactions | 2 |
| Genome length (bp) | 68324 |
| Character of genome ends | Circularly Permuted |
| GC Content | 66.5% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | B |
| Subcluster | B1 |
| Cluster Life Cycle | Lytic |
| Other Cluster Members |
|
| Annotating Institution | Unknown or unassigned |
| Annotation Status | In GenBank |
| Plaque Notes | Plaque shape relatively consistent. Plaques were all circular with varying sizes due to different dilutions.
Clear, translucent plaque. |
| Morphotype | Siphoviridae |
| Number of Genes | 102 |
| Number of tRNAs | 0 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Submitted Minimal DNA Master File | Download |
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | MF155947 |
| Refseq Number | None yet |
| Published in Paper | Pope, Bowman, Russell, et al 2015, eLIFE: Whole genome comparison of a large collection of mycobacteriophages reveals a continuum of phage genetic diversity |
| Archiving Info |
| Archiving status |
Archived |
| SEA Designator |
2011WUSLlemonsliceKH |
| SEA Lysate Titer |
8.86e8 |
| Date of SEA Lysate Titering |
Sep 27, 2011 |
| Pitt Freezer Box# |
31 |
| Pitt Freezer Box Grid# |
3 |
| Available Files |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| EM Picture | Download |
| GenBank File for Phamerator | Download |