| Detailed Information for Phage LestyG |
| Discovery Information |
| Isolation Host | Mycobacterium smegmatis mc²155 |
| Found By | Caleb Manu |
| Year Found | 2020 |
| Location Found | Farmville, VA United States |
| Finding Institution | Hampden-Sydney College |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | 30°C |
| GPS Coordinates | 37.24 N, 78.45 W Map |
| Discovery Notes | This bacteriophage was found in the soil of the Aztec grass in front of the Rhetoric Studio on the Hampden-Sydney College campus. |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Aug 25, 2020 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina |
| Approximate Shotgun Coverage | 394 |
| Genome length (bp) | 68198 |
| Character of genome ends | Circularly Permuted |
| GC Content | 67.6% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | B |
| Subcluster | B3 |
| Cluster Life Cycle | Lytic |
| Other Cluster Members |
|
| Annotating Institution | Hampden-Sydney College |
| Annotation Status | In GenBank |
| Plaque Notes | The bacteriophage thrived in both 37 degree Celsius and 30 degree Celsius incubator. The growth rate in 37 degrees Celsius was faster (fully developed in 1 day) whiles the growth rate in 30 degrees Celsius was slower (fully developed within 2 to 3 days). The plaques of the bacteriophage was moderate. |
| Morphotype | Siphoviridae |
| Number of Genes | 101 |
| Number of tRNAs | 0 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Submitted Annotation in DNA Master Format | Download |
| Submitted Minimal DNA Master File | Download |
| Author List | Download |
| Annotation Cover Sheet | Download |
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | OK318960 |
| Refseq Number | None yet |
| Archiving Info |
| Archiving status |
Not in Pitt Archives |
| Available Files |
| Plaque Picture | Download |
| EM Picture | Download |
| GenBank File for Phamerator | Download |
