| Detailed Information for Phage LilSmirk |
| Discovery Information |
| Isolation Host | Mycobacterium smegmatis mc²155 |
| Found By | Sharanya Deshpande |
| Year Found | 2024 |
| Location Found | Richmond, VA United States of America |
| Finding Institution | Virginia Commonwealth University |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | Not entered |
| GPS Coordinates | 37.545641 N, 77.454101 W Map |
| Discovery Notes | The first spot test of the sample resulted in a positive result. I went through two successful rounds of purification, followed by one failed purification, and one failed amplification. |
| Naming Notes | It was named after TEM image, phage was in the shape of a smile. |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Jan 10, 2025 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina |
| Sequencer Used | Illumina NextSeq 1000 |
| Read Type | Single-end reads |
| Read Length | 100 bp |
| Approximate Shotgun Coverage | 2523 |
| Genome length (bp) | 67360 |
| Character of genome ends | Circularly Permuted |
| GC Content | 68.9% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | B |
| Subcluster | B2 |
| Cluster Life Cycle | Lytic |
| Other Cluster Members |
|
| Annotating Institution | Virginia Commonwealth University |
| Annotation Status | Awaiting_QC |
| Plaque Notes | Plaque morphology is round clear circles, approximately 1 mm in diameter. |
| Morphotype | Siphoviridae |
| Number of Genes | 92 |
| Number of tRNAs | 0 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Submitted Annotation in DNA Master Format | Download |
| Submitted Minimal DNA Master File | Download |
| Author List | Download |
| Annotation Cover Sheet | Download |
| Publication Info |
| Uploaded to GenBank? | No |
| GenBank Accession | None yet |
| Refseq Number | None yet |
| Archiving Info |
| Archiving status |
Archived |
| SEA Lysate Titer |
1.04 10^9 |
| Pitt Freezer Box# |
200 |
| Pitt Freezer Box Grid# |
H12 |
| Available Files |
| Plaque Picture | Download |
| EM Picture | Download |
