| Detailed Information for Phage LittleDigger |
| Discovery Information |
| Isolation Host | Mycobacterium smegmatis mc²155 |
| Found By | J Mayoral, P Waikel, L Becerra Reymundo |
| Year Found | 2025 |
| Location Found | Miami, FL United States |
| Finding Institution | Florida International University |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | 37°C |
| GPS Coordinates | 25.74496 N, 80.30521 W Map |
| Discovery Notes | Found in soil near the drip of the condensation line of the air conditioner. Soil was moist with some sparse weeds and grass. There was a biofilm on the surface of the soil. |
| Naming Notes | Dedicated to Liam Mayoral, who is a good reminder of the child like curiosity in all of us. |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Dec 17, 2025 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina |
| Sequencer Used | Illumina NextSeq 1000 |
| Read Type | Single-end reads |
| Read Length | 100 bp |
| Approximate Shotgun Coverage | 511 |
| Genome length (bp) | 70752 |
| Character of genome ends | Circularly Permuted |
| GC Content | 69.0% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | B |
| Subcluster | B4 |
| Cluster Life Cycle | Lytic |
| Other Cluster Members |
|
| Annotating Institution | Florida International University |
| Annotation Status | Being Annotated (Expected completion by 5/1/2026) |
| Number of Genes | 98 |
| Number of tRNAs | 0 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Publication Info |
| Uploaded to GenBank? | No |
| GenBank Accession | None yet |
| Refseq Number | None yet |
| Archiving Info |
| Archiving status |
Not in Pitt Archives |