| Detailed Information for Phage LittleMinkey |
| Discovery Information |
| Isolation Host | Mycobacterium smegmatis mc²155 |
| Found By | Dimpho Lukhele |
| Year Found | 2025 |
| Location Found | Cape Town, South Africa |
| Finding Institution | TASK South Africa |
| Program | Unavailable |
| From enriched soil sample? | No |
| Isolation Temperature | 37°C |
| GPS Coordinates | 33.892667 S, 18.556412 E Map |
| Discovery Notes | Soil was sampled approximately 2-3cm from the base of an outdoor plant, soil was moist, well aerated and rich in organic matter. It was then stored in a 40ml airtight specimen jar 24hours before phage enrichment with Mycobacterium Smegmatis. |
| Naming Notes | Phage name is a combination of my nickname (Little Kenky), and my late little sister's name (Minnie) who I never got to see, both names special to my mom so my phage honours us as her girls and carries comfort. |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Aug 11, 2025 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina |
| Sequencer Used | Illumina NextSeq 1000 |
| Read Type | Single-end reads |
| Read Length | 100 bp |
| Approximate Shotgun Coverage | 2172 |
| Genome length (bp) | 70310 |
| Character of genome ends | Circularly Permuted |
| GC Content | 70.2% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | B |
| Subcluster | B13 |
| Cluster Life Cycle | Lytic |
| Other Cluster Members |
|
| Annotating Institution | TASK South Africa |
| Annotation Status | Being Annotated (Expected completion by 2/11/2026) |
| Plaque Notes | Turbid plaques |
| Number of Genes | 108 |
| Number of tRNAs | 1 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Publication Info |
| Uploaded to GenBank? | No |
| GenBank Accession | None yet |
| Refseq Number | None yet |
| Archiving Info |
| Archiving status |
Not in Pitt Archives |
| Available Files |
| Plaque Picture | Download |
| EM Picture | Download |
