The Actinobacteriophage Database

Mycobacterium phage Lolalove

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Detailed Information for Phage Lolalove
Discovery Information
Isolation Host	Mycobacterium smegmatis mc²155
Found By	Hannah Marie Rozier
Year Found	2018
Location Found	Charleston, SC USA
Finding Institution	College of Charleston
Program	Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?	Yes
Isolation Temperature	37°C
GPS Coordinates	32.787021 N, 79.934143 W Map
Discovery Notes	Found at a Shell gas station. The soil was dark and wet. Initially discovered and named by Hannah Marie Rozier. Additional work on sample was done by Cody Johnson and Stephanie Jackson.
Naming Notes	Named after my dog.
Sequencing Information
Sequencing Complete?	Yes
Date Sequencing Completed	Jan 10, 2019
Sequencing Facility	Pittsburgh Bacteriophage Institute
Shotgun Sequencing Method	Illumina
Sequencer Used	Illumina MiSeq
Read Type	Single-end reads
Read Length	150 bp
Approximate Shotgun Coverage	1193
Genome length (bp)	71111
Character of genome ends	Circularly Permuted
GC Content	68.9%
Sequencing Notes	In this sample, there was another Cluster B4 genome present. Relative coverages of discrepant contigs indicate that about 80% of the DNA belongs to the phage which we've posted as the official Lolalove, while 20% belongs to the second B4 phage. The lysate sample this DNA came from might be similarly mixed.
Fasta file available?	Yes: Download fasta file
Characterization
Cluster	B
Subcluster	B4
Cluster Life Cycle	Lytic
Other Cluster Members	Click to View AbsoluteMadLad (B1) ABU (B1) Adriana (B1) Aelin (B1) AltPhacts (B1) Altwerkus (B1) Anderson (B1) Andre (B1) AngelCake (B1) Antonia (B1) Apizium (B1) Ashraf (B1) Badfish (B1) Banjo (B1) Basato (B1) BatteryCK (B1) Beaglebox (B1) Bishoperium (B1) BlackStallion (B1) BlueHusk (B1) Bluephacebaby (B1) Boehler (B1) Brilliant (B1) Buckeye (B1) Burr (B1) CamL (B1) CampRoach (B1) Cannibal (B1) Carthage (B1) Caspiboi (B1) Chaelin (B1) Chah (B1) Charles1 (B1) CharlieGBrown (B1) CheetO (B1) Cher (B1) Childish (B1) Chipette (B1) Chorkpop (B1) Chunky (B1) Chute (B1) Cobra (B1) Colbert (B1) Coletti (B1) Cornbread (B1) Cornobble (B1) Cosmolli16 (B1) Craff (B1) Crownjwl (B1) DaddyDaniels (B1) Daffy (B1) Daka (B1) Dati (B1) DelRivs (B1) Derpp (B1) Dice (B1) Dingo (B1) Dione (B1) DirtJuice (B1) Doddsville (B1) DoesntMatter (B1) DonSanchon (B1) DuchessDung (B1) Duggie (B1) Durga (B1) ElvisPhasley (B1) Emiris (B1) EmpTee (B1) Eremos (B1) Etaye (B1) Eugenia (B1) Fang (B1) FluffyNinja (B1) Fozzie (B1) Frankicide (B1) FremontTroll (B1) Freya (B1) FriarPreacher (B1) Fringe (B1) FugateOSU (B1) Gareth (B1) GeneCoco (B1) Giraffe (B1) Gophee (B1) Grand2040 (B1) Gwennie1 (B1) Gwilliam (B1) Gyarad (B1) Haimas (B1) Haleema (B1) Hamish (B1) Hartsy (B1) Harvey (B1) Held (B1) HenryJackson (B1) Hertubise (B1) Hetaeria (B1) HighStump (B1) Hocus (B1) Horchata (B1) HSavage (B1) ImtiyazSitla (B1) Inchworm (B1) Inverness (B1) Iridessa (B1) Iridoclysis (B1) IsaacEli (B1) JacAttac (B1) JakeO (B1) JangoPhett (B1) JDog (B1) Jesabah (B1) Jillium (B1) Jiminy (B1) Jiraiya (B1) Kahve (B1) Kailash (B1) Katniss (B1) Keitherie (B1) Khan1 (B1) Kikipoo (B1) Kimbrough (B1) KingTut (B1) KingVeVeVe (B1) KlimbOn (B1) Kloppinator (B1) KLucky39 (B1) Kwadwo (B1) Kwksand96 (B1) Labeouficaum (B1) LasagnaCat (B1) Lasso (B1) LeeLot (B1) Lego3393 (B1) Legolas (B1) Lehmann (B1) LeiMonet (B1) LemonSlice (B1) Longacauda (B1) Lopsy (B1) LostAndPhound (B1) LuckyMarjie (B1) Lulumae (B1) Lulwa (B1) Lumine (B1) Mag7 (B1) Magic8 (B1) Mana (B1) Manad (B1) Maru (B1) Maskar (B1) Matalotodo (B1) Mcshane (B1) Mecca (B1) Megamind20 (B1) Megatron (B1) Melc17 (B1) MelsMeow (B1) Mesh1 (B1) Mesmerelda (B1) MichaelPhcott (B1) Mikota (B1) MiniBoss (B1) MitKao (B1) Morgushi (B1) Morty (B1) Mosaic (B1) MRabcd (B1) MrPhizzler (B1) Mulan (B1) Murdoc (B1) Mutante (B1) Nacho (B1) Nathan (B1) Newman (B1) Nicole21 (B1) Numberten (B1) Nyala (B1) Olak (B1) Oline (B1) Olive (B1) OliverWalter (B1) Omniscient (B1) Oosterbaan (B1) Orfeu (B1) Orion (B1) Orwigg (B1) OSmaximus (B1) Pacifista (B1) PG1 (B1) Phamished (B1) Phareon (B1) PhatCats2014 (B1) PhatLouie (B1) PhenghisKhan (B1) Pherdinand (B1) Phergie (B1) Phipps (B1) Phleuron (B1) PhrankReynolds (B1) PhrodoBaggins (B1) Phunky (B1) Piglet (B1) PinheadLarry (B1) Pinkman (B1) Pipsqueak (B1) Placalicious (B1) Plata (B1) Plmatters (B1) Podrick (B1) Pops (B1) Potter (B1) Prickles (B1) ProfessorX (B1) Puhltonio (B1) QueenBeane (B1) Quisquiliae (B1) RedMaple (B1) Ricotta (B1) Riggan (B1) Rimu (B1) Riverton (B1) Robyn (B1) Roliet (B1) Roscoe (B1) Roy17 (B1) Samaymay (B1) SassyCat97 (B1) Schadenfreude (B1) Schueller (B1) Scoot17C (B1) Scrick (B1) SDcharge11 (B1) Selr12 (B1) Serendipity (B1) Serpentine (B1) Sheila (B1) ShiVal (B1) Sigman (B1) SilverBell (B1) Simielle (B1) SirBeauregard (B1) Skippy (B1) Slatt (B1) Soile (B1) Solosis (B1) Sophia (B1) Soto (B1) Spartan300 (B1) Squid (B1) Squiggle (B1) Struggle (B1) Suffolk (B1) Surely (B1) Swiphy (B1) Swish (B1) TallGrassMM (B1) Telesworld (B1) Thora (B1) ThreeOh3D2 (B1) Thunderbird (B1) Timmi (B1) TomBombadil (B1) Tomlarah (B1) Toni (B1) TooFast2Furius (B1) Tooj (B1) Triad (B1) True (B1) Trypo (B1) TyrionL (B1) UAch1 (B1) UncleHowie (B1) Usavi (B1) Vaishali24 (B1) Valjean (B1) Vaticameos (B1) Veritas (B1) Virapocalypse (B1) Virgeve (B1) Vista (B1) Vivaldi (B1) Vortex (B1) Wallhey (B1) Waterdiva (B1) Weher20 (B1) Windsor (B1) Xavier (B1) Yoshand (B1) YouGoGlencoco (B1) Zaider (B1) Zelda (B1) Zonia (B1) Imvubu (B10) Indlovu (B11) LilMcDreamy (B12) BirdsNest (B13) Hashim76 (B13) LittleMinkey (B13) Zenteno07 (B13) Allegro (B2) Arbiter (B2) Ares (B2) Bananafish (B2) Blocker23 (B2) Boyle (B2) Brownie5 (B2) Calamitous (B2) Coffee (B2) Eaglehorse (B2) Faze9 (B2) FrenchFry (B2) Glass (B2) Godines (B2) Hedgerow (B2) Holeinone (B2) ItsyBitsy1 (B2) Kaleb (B2) Kheth (B2) Kolhan (B2) Lars (B2) Laurie (B2) Lephleur (B2) LilSmirk (B2) LizLemon (B2) MasterPo (B2) Opia (B2) Phantasmagoria (B2) PrinceCharming (B2) Qyrzula (B2) Rhinoforte (B2) Rosebush (B2) Sabella (B2) TA17A (B2) Tinciduntsolum (B2) Tres (B2) Vic9 (B2) West99 (B2) Abinghost (B3) Akoma (B3) Athena (B3) Audrey (B3) Baloo (B3) Ben11 (B3) Bernardo (B3) Briakila (B3) Casbah (B3) ChaChing (B3) Chalmers (B3) Chandler (B3) Chentzyk (B3) Compostia (B3) Corofin (B3) Daisy (B3) Deenasa (B3) Devonte (B3) Gadjet (B3) Gervas (B3) GlenHope (B3) Glubnar (B3) Halfpint (B3) HarveySr (B3) Heathcliff (B3) Jackstina (B3) Kamiyu (B3) Kronus (B3) LestyG (B3) Marley1013 (B3) Marlia (B3) MmasiCarm (B3) Mortcellus (B3) Morty007 (B3) Neos5 (B3) Nozo (B3) Obutu (B3) Olivio (B3) OrangeOswald (B3) Phaedrus (B3) Phayeta (B3) Philly (B3) Phlyer (B3) Pipefish (B3) RagingRooster (B3) Rita1961 (B3) RomaT (B3) Shenandoah (B3) SlippinJimmy (B3) SwainyDoc (B3) SynergyX (B3) TinyTerror (B3) Tydolla (B3) Yahalom (B3) Yinz (B3) Ahwei (B4) AlanGrant (B4) Antihero (B4) Apex (B4) Austelle (B4) BobbyK (B4) BrownCNA (B4) ChrisnMich (B4) Cooper (B4) Epah (B4) Fanaw (B4) Fortunato (B4) Frederick (B4) GinPorsche (B4) Hally898 (B4) Hangman (B4) Heath (B4) Hydro (B4) JaguarMi (B4) JAMaL (B4) Lambano (B4) Lolalove (B4) Magpie (B4) Mithril (B4) Mudslide (B4) Nanao (B4) Nigel (B4) Poster (B4) Prince (B4) QueenPie (B4) RawrgerThat (B4) Stinger (B4) Vincenzo (B4) VioletZ (B4) Waleliano (B4) Xincheng (B4) Zemanar (B4) Acadian (B5) Baee (B5) DigitDog (B5) Donny (B5) EastView9101 (B5) KarasumaRum (B5) Mysterious (B5) Nangang (B5) Nehemiah (B5) Phelemich (B5) Reprobate (B5) Rich (B5) Rita130 (B5) Serendipitous (B5) Shenyu (B5) SirJeffery (B5) StinkyVeggy (B5) Suigeneris (B5) 39HC (B6) 40BC (B6) ForDig (B6) Hosp (B6) Jolie1 (B6) KayaCho (B6) Saguaro (B7) Thonko (B8) CRB2 (B9) Funsized (B9) Knocker (B9) PenguinLover67 (B9) Quesadilla (B9) Rinkes (B9)
Annotating Institution	College of Charleston
Annotation Status	In GenBank
Plaque Notes	TEM measurements by Christine Byrum (all photos collected for TEM from Jackson, Rozier, Johnson): Capsid Diameter = 71.43 - 78.57nm, Avg = 76.83nm Tail Length = 310 - 356.92 nm, Avg = 329.34nm Tail Diameter = 10.94 - 12.86nm, Avg = 12.8nm TEM measurements from Hannah Marie Rozier: Head Diameter = 87.5 nm Tail Length = 365.6 nm Tail Diameter = 12.5 nm TEM measurements from Stephanie Jackson: Head Diameter = 75 nm Tail Length = 337.5 nm Tail Diameter = 16.7 nm
Morphotype	Siphoviridae
Number of Genes	99
Number of tRNAs	0
Number of tmRNAs	0
Has been Phamerated?	Yes
Gene List	Click to View Lolalove_1 (ParB-like nuclease domain protein) Lolalove_2 (hydroxylase) Lolalove_3 (nucleotide pyrophosphohydrolase) Lolalove_4 (adenylate kinase) Lolalove_5 (terminase) Lolalove_6 Lolalove_7 (RuvC-like resolvase) Lolalove_8 Lolalove_9 (portal protein) Lolalove_10 (capsid morphogenesis protein) Lolalove_11 Lolalove_12 (major capsid hexamer protein) Lolalove_13 (major capsid pentamer protein) Lolalove_14 Lolalove_15 Lolalove_16 Lolalove_17 Lolalove_18 (major tail protein) Lolalove_19 Lolalove_20 (head-to-tail adaptor) Lolalove_21 Lolalove_22 Lolalove_23 (tail assembly chaperone) Lolalove_24 Lolalove_25 (tape measure protein) Lolalove_26 (minor tail protein) Lolalove_27 (minor tail protein) Lolalove_28 (minor tail protein) Lolalove_29 (minor tail protein) Lolalove_30 (minor tail protein) Lolalove_31 Lolalove_32 Lolalove_33 Lolalove_34 Lolalove_35 Lolalove_36 Lolalove_37 Lolalove_38 Lolalove_39 Lolalove_40 (helix-turn-helix DNA binding domain protein) Lolalove_41 (helix-turn-helix DNA binding domain protein) Lolalove_42 Lolalove_43 Lolalove_44 (lysin A) Lolalove_45 (lysin B) Lolalove_46 Lolalove_47 (exonuclease) Lolalove_48 (DNA helicase) Lolalove_49 Lolalove_50 Lolalove_51 Lolalove_52 (DNA primase/polymerase) Lolalove_53 Lolalove_54 (DNA polymerase I) Lolalove_55 Lolalove_56 Lolalove_57 Lolalove_58 Lolalove_59 Lolalove_60 Lolalove_61 Lolalove_62 (ribbon-helix-helix DNA binding domain protein) Lolalove_63 Lolalove_64 Lolalove_65 Lolalove_66 Lolalove_67 Lolalove_68 Lolalove_69 (helix-turn-helix DNA binding domain protein) Lolalove_70 Lolalove_71 Lolalove_72 Lolalove_73 (HNH endonuclease) Lolalove_74 Lolalove_75 Lolalove_76 Lolalove_77 (HNH endonuclease) Lolalove_78 Lolalove_79 Lolalove_80 Lolalove_81 Lolalove_82 Lolalove_83 Lolalove_84 Lolalove_85 Lolalove_86 (HicA-like toxin) Lolalove_87 Lolalove_88 Lolalove_89 Lolalove_90 Lolalove_91 Lolalove_92 Lolalove_93 Lolalove_94 Lolalove_95 Lolalove_96 Lolalove_97 Lolalove_98 Lolalove_99
Submitted Annotation in DNA Master Format	Download
Submitted Minimal DNA Master File	Download
Author List	Download
Annotation Cover Sheet	Download
Publication Info
Uploaded to GenBank?	Yes
GenBank Accession	MT818419
Refseq Number	None yet
Published in Paper	Byrum et al 2021, Microbiology Resource Announcements: Complete Genome Sequence of the Cluster B4 Mycobacteriophage Lolalove, Isolated in Charleston, South Carolina
Archiving Info
Archiving status	Archived
Pitt Freezer Box#	93
Pitt Freezer Box Grid#	E3
Available Files
Restriction Digest Picture	Download
EM Picture	Download
GenBank File for Phamerator	Download

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