| Detailed Information for Phage Manad |
| Discovery Information |
| Isolation Host | Mycobacterium smegmatis mc²155 |
| Found By | Matt Nelson and Andie Doak |
| Year Found | 2012 |
| Location Found | Boulder, CO USA |
| Finding Institution | University of Colorado Boulder |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | Not entered |
| GPS Coordinates | 40.002869 N, 104.261003 W Map |
| Discovery Notes | Soil from the bottom of a stream. 3cm below water line, top 1cm of soil. Surrounded by plants, grassy, wet dirt. East side of Kittridge pond at head of creek. 22.8°C; 9-9-2012. |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Jan 7, 2013 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Ion Torrent |
| Approximate Shotgun Coverage | 900 |
| Genome length (bp) | 68807 |
| Character of genome ends | Circularly Permuted |
| GC Content | 66.4% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | B |
| Subcluster | B1 |
| Cluster Life Cycle | Lytic |
| Other Cluster Members |
|
| Annotating Institution | Unknown or unassigned |
| Annotation Status | In GenBank |
| Plaque Notes | Approximately 1-3 mm diameter, clear, 48 hr incubation time at 37°C |
| Morphotype | Siphoviridae |
| Number of Genes | 101 |
| Number of tRNAs | 0 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | KJ595576 |
| Refseq Number | NC_024363 |
| Published in Paper | Pope, Bowman, Russell, et al 2015, eLIFE: Whole genome comparison of a large collection of mycobacteriophages reveals a continuum of phage genetic diversity |
| Archiving Info |
| Archiving status |
Archived |
| SEA Lysate Titer |
3.0e10 |
| Date of SEA Lysate Titering |
Oct 22, 2012 |
| Pitt Freezer Box# |
40 |
| Pitt Freezer Box Grid# |
50 |
| Available Files |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| EM Picture | Download |
