Mycobacterium phage MelsMeow
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Detailed Information for Phage MelsMeow
Discovery Information
Isolation HostMycobacterium smegmatis mc²155
Found BySara Dixson & Melody Iacino
Year Found2016
Location FoundRock Hill, SC USA
Finding InstitutionWinthrop University
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?Yes
Isolation Temperature24°C
GPS Coordinates34.9371 N, 81.032 W Map
Discovery NotesThis phage was collected at night, approx. 75 degrees Fahrenheit,dry soil,only 1-2 inches deep.Sara Dixson collected the phage and I picked a plaque off of her 10^-7 plate. There were a few plaques, all clear, about 2 millimeters wide,and spaced randomly throughout the plate.
Titer - 2.84 x 10^10
Naming NotesThis phage came from Hardin Family Garden at Winthrop University. Will rename later if necessary
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedAug 17, 2021
Sequencing FacilityPittsburgh Bacteriophage Institute
Shotgun Sequencing MethodIllumina Sequencing
Approximate Shotgun Coverage646
Genome length (bp)68563
Character of genome endsCircularly Permuted
GC Content66.4%
Fasta file available?Yes: Download fasta file
Characterization
ClusterB
SubclusterB1
Cluster Life CycleLytic
Other Cluster Members

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Lego3393 (B1)

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Matalotodo (B1)

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Yahalom (B3)

Yinz (B3)

Ahwei (B4)

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Austelle (B4)

BobbyK (B4)

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ChrisnMich (B4)

Cooper (B4)

Epah (B4)

Fanaw (B4)

Fortunato (B4)

Frederick (B4)

GinPorsche (B4)

Hally898 (B4)

Hangman (B4)

Heath (B4)

Hydro (B4)

JaguarMi (B4)

JAMaL (B4)

Lambano (B4)

Lolalove (B4)

Magpie (B4)

Mithril (B4)

Mudslide (B4)

Nanao (B4)

Nigel (B4)

Poster (B4)

Prince (B4)

QueenPie (B4)

RawrgerThat (B4)

Stinger (B4)

Vincenzo (B4)

VioletZ (B4)

Waleliano (B4)

Xincheng (B4)

Zemanar (B4)

Acadian (B5)

Baee (B5)

DigitDog (B5)

Donny (B5)

EastView9101 (B5)

KarasumaRum (B5)

Mysterious (B5)

Nangang (B5)

Nehemiah (B5)

Phelemich (B5)

Reprobate (B5)

Rich (B5)

Serendipitous (B5)

Shenyu (B5)

SirJeffery (B5)

StinkyVeggy (B5)

Suigeneris (B5)

39HC (B6)

40BC (B6)

Hosp (B6)

Jolie1 (B6)

KayaCho (B6)

Saguaro (B7)

Thonko (B8)

CRB2 (B9)

Funsized (B9)

Knocker (B9)

PenguinLover67 (B9)

Quesadilla (B9)

Rinkes (B9)

Annotating InstitutionWinthrop University
Annotation StatusIn GenBank
MorphotypeSiphoviridae
Number of Genes100
Number of tRNAs0
Number of tmRNAs0
Has been Phamerated?Yes
Gene List

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MelsMeow_1
(adenylate kinase)

MelsMeow_2
(teminase)

MelsMeow_3

MelsMeow_4

MelsMeow_5

MelsMeow_6
(RuvC-like resolvase)

MelsMeow_7

MelsMeow_8
(portal protein)

MelsMeow_9
(capsid maturation protease and MuF-like fusion protein)

MelsMeow_10

MelsMeow_11
(major capsid hexamer protein)

MelsMeow_12
(major capsid pentamer protein)

MelsMeow_13

MelsMeow_14

MelsMeow_15

MelsMeow_16

MelsMeow_17
(major tail protein)

MelsMeow_18

MelsMeow_19
(queuine tRNA-ribosyltransferase)

MelsMeow_20

MelsMeow_21
(head-to-tail adaptor)

MelsMeow_22

MelsMeow_23

MelsMeow_24
(tail assembly chaperone)

MelsMeow_25

MelsMeow_26

MelsMeow_27
(tape measure protein)

MelsMeow_28
(minor tail protein)

MelsMeow_29
(minor tail protein)

MelsMeow_30
(minor tail protein)

MelsMeow_31
(minor tail protein)

MelsMeow_32
(minor tail protein)

MelsMeow_33

MelsMeow_34

MelsMeow_35

MelsMeow_36

MelsMeow_37

MelsMeow_38

MelsMeow_39

MelsMeow_40

MelsMeow_41

MelsMeow_42

MelsMeow_43

MelsMeow_44
(helix-turn-helix DNA binding domain protein)

MelsMeow_45
(helix-turn-helix DNA binding domain protein)

MelsMeow_46

MelsMeow_47

MelsMeow_48
(lysin A)

MelsMeow_49
(lysin B)

MelsMeow_50

MelsMeow_51

MelsMeow_52
(DNA helicase)

MelsMeow_53

MelsMeow_54

MelsMeow_55

MelsMeow_56

MelsMeow_57

MelsMeow_58
(DNA primase/helicase)

MelsMeow_59

MelsMeow_60

MelsMeow_61
(DNA polymerase I)

MelsMeow_62

MelsMeow_63

MelsMeow_64

MelsMeow_65

MelsMeow_66

MelsMeow_67

MelsMeow_68

MelsMeow_69
(DNA binding protein)

MelsMeow_70

MelsMeow_71

MelsMeow_72

MelsMeow_73

MelsMeow_74

MelsMeow_75

MelsMeow_76

MelsMeow_77

MelsMeow_78

MelsMeow_79

MelsMeow_80

MelsMeow_81

MelsMeow_82

MelsMeow_83
(HNH endonuclease)

MelsMeow_84

MelsMeow_85

MelsMeow_86

MelsMeow_87

MelsMeow_88

MelsMeow_89

MelsMeow_90

MelsMeow_91

MelsMeow_92

MelsMeow_93

MelsMeow_94

MelsMeow_95

MelsMeow_96

MelsMeow_97

MelsMeow_98

MelsMeow_99

MelsMeow_100

Submitted Minimal DNA Master FileDownload
Publication Info
Uploaded to GenBank?Yes
GenBank AccessionON456330
Refseq NumberNone yet
Archiving Info
Archiving status Archived
Pitt Freezer Box# 43
Pitt Freezer Box Grid# B4
Available Files
Plaque PictureDownload
Restriction Digest PictureDownload
EM PictureDownload
GenBank File for PhameratorDownload