Mycobacterium phage Mutante
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Detailed Information for Phage Mutante
Discovery Information
Isolation HostMycobacterium smegmatis mc²155
Found ByHannah Elizabeth Miller
Year Found2015
Location FoundBowling Green, KY USA
Finding InstitutionWestern Kentucky University
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?Yes
Isolation Temperature30°C
GPS Coordinates36.986283 N, 86.456568 W Map
Discovery NotesSoil sample collected from Western Kentucky University's campus on September 1, 2015. This area contained moist soil, and very little decay. Smokers often smoke in this area, and it is a heavily traveled.

This phage has clear plaque mutant tendencies. The beginning plaques differ greatly in size from the plaques towards the end of my discovery.
Naming NotesThis phage has some clear plaque mutant tendencies; therefore, originally earning the name "Mutant." Since the term mutant has a specific scientific meaning and would be confusing when discussing the phage "Mutant," I chose to name my phage Mutante, which is mutant in Spanish.
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedFeb 4, 2016
Sequencing FacilityPittsburgh Bacteriophage Institute
Shotgun Sequencing MethodIllumina
Sequencer UsedIllumina MiSeq
Read TypeSingle-end reads
Read Length150 bp
Approximate Shotgun Coverage2177
Genome length (bp)68494
Character of genome endsCircularly Permuted
GC Content66.5%
Fasta file available?Yes: Download fasta file
Characterization
ClusterB
SubclusterB1
Cluster Life CycleLytic
Lysogeny NotesNever had turbid plaques.
Other Cluster Members
Annotating InstitutionWestern Kentucky University
Annotation StatusIn GenBank
Plaque NotesBeginning plaques: large clear plaques, organically shaped, very lytic
Plaques during the middle of the process: very small, lytic organically shaped, tend to grow in a swirl pattern despite not being meant to grow that way
MorphotypeSiphoviridae
Number of Genes103
Number of tRNAs0
Number of tmRNAs0
Has been Phamerated?Yes
Gene List
Submitted Annotation in DNA Master FormatDownload
Submitted Minimal DNA Master FileDownload
Author ListDownload
Annotation Cover SheetDownload
Publication Info
Uploaded to GenBank?Yes
GenBank AccessionMH399780
Refseq NumberNone yet
Archiving Info
Archiving status Archived
SEA Designator 2015WEKUMutanteHEM
SEA Lysate Titer 1.6x10^10
Date of SEA Lysate Titering Oct 20, 2015
Pitt Freezer Box# 27
Pitt Freezer Box Grid# F5
Available Files
Plaque PictureDownload
Restriction Digest PictureDownload
EM PictureDownload
GenBank File for PhameratorDownload