| Discovery Information |
| Isolation Host | Mycobacterium smegmatis mc²155 |
| Found By | Hannah Elizabeth Miller |
| Year Found | 2015 |
| Location Found | Bowling Green, KY USA |
| Finding Institution | Western Kentucky University |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | 30°C |
| GPS Coordinates | 36.986283 N, 86.456568 W Map |
| Discovery Notes | Soil sample collected from Western Kentucky University's campus on September 1, 2015. This area contained moist soil, and very little decay. Smokers often smoke in this area, and it is a heavily traveled.
This phage has clear plaque mutant tendencies. The beginning plaques differ greatly in size from the plaques towards the end of my discovery. |
| Naming Notes | This phage has some clear plaque mutant tendencies; therefore, originally earning the name "Mutant." Since the term mutant has a specific scientific meaning and would be confusing when discussing the phage "Mutant," I chose to name my phage Mutante, which is mutant in Spanish. |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Feb 4, 2016 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina |
| Sequencer Used | Illumina MiSeq |
| Read Type | Single-end reads |
| Read Length | 150 bp |
| Approximate Shotgun Coverage | 2177 |
| Genome length (bp) | 68494 |
| Character of genome ends | Circularly Permuted |
| GC Content | 66.5% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | B |
| Subcluster | B1 |
| Cluster Life Cycle | Lytic |
| Lysogeny Notes | Never had turbid plaques. |
| Other Cluster Members |
|
| Annotating Institution | Western Kentucky University |
| Annotation Status | In GenBank |
| Plaque Notes | Beginning plaques: large clear plaques, organically shaped, very lytic
Plaques during the middle of the process: very small, lytic organically shaped, tend to grow in a swirl pattern despite not being meant to grow that way |
| Morphotype | Siphoviridae |
| Number of Genes | 103 |
| Number of tRNAs | 0 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Submitted Annotation in DNA Master Format | Download |
| Submitted Minimal DNA Master File | Download |
| Author List | Download |
| Annotation Cover Sheet | Download |
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | MH399780 |
| Refseq Number | None yet |
| Archiving Info |
| Archiving status |
Archived |
| SEA Designator |
2015WEKUMutanteHEM |
| SEA Lysate Titer |
1.6x10^10 |
| Date of SEA Lysate Titering |
Oct 20, 2015 |
| Pitt Freezer Box# |
27 |
| Pitt Freezer Box Grid# |
F5 |
| Available Files |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| EM Picture | Download |
| GenBank File for Phamerator | Download |
