| Detailed Information for Phage PhatCats2014 |
| Discovery Information |
| Isolation Host | Mycobacterium smegmatis mc²155 |
| Found By | AP Biology Class |
| Year Found | 2013 |
| Location Found | Cleveland, OH USA |
| Finding Institution | St. Ignatius High School |
| Program | Phage Hunters Integrating Research and Education |
| From enriched soil sample? | No |
| Isolation Temperature | Not entered |
| GPS Coordinates | 41.518362 N, 81.527966 W Map |
| Discovery Notes | Soil from geranium pot placed in Winogradsky column for 30 days |
| Naming Notes | School mascot is Wildcats. Phat is in the spirit of phage. Senior class 2014 |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Feb 10, 2015 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina |
| Sequencer Used | Illumina MiSeq |
| Read Type | Single-end reads |
| Read Length | 150 bp |
| Approximate Shotgun Coverage | 2410 |
| Genome length (bp) | 69000 |
| Character of genome ends | Circularly Permuted |
| GC Content | 66.5% |
| Sequencing Notes | Exactly 69000 base pairs long! |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | B |
| Subcluster | B1 |
| Cluster Life Cycle | Lytic |
| Other Cluster Members |
|
| Annotating Institution | Unknown or unassigned |
| Annotation Status | In GenBank |
| Morphotype | Siphoviridae |
| Number of Genes | 101 |
| Number of tRNAs | 0 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | KX369585 |
| Refseq Number | None yet |
| Archiving Info |
| Archiving status |
Archived |
| SEA Lysate Titer |
1.2x10^11 pfu/mL |
| Date of SEA Lysate Titering |
Oct 10, 2014 |
| Available Files |
| Plaque Picture | Download |
| Final DNAMaster File | Download |
| GenBank File for Phamerator | Download |
