| Detailed Information for Phage Phergie |
| Discovery Information |
| Isolation Host | Mycobacterium smegmatis mc²155 |
| Found By | Divya Balchander & Kristofer Apellido |
| Year Found | 2015 |
| Location Found | Philadelphia, PA USA |
| Finding Institution | Drexel University |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | Not entered |
| GPS Coordinates | 39.95681 N, 75.18959 W Map |
| Discovery Notes | Collected in the Drexel University College of Computing and Informatics Garden, by the cascading fountain.
Conditions during Collection:
Approximately 72 °F with little to mild humidity
sample collected 2 inches below the surface from moist soil, surrounded by foliage.
|
| Naming Notes | Our phage is inspired by the artist Fergie.
"If you ain't got no plaques take yo' broke plate home
You say: If you ain't got no plaques take yo'broke plate home
S-E-A-P-H-A-G-E-S, yeah S-E-A-P-H-A-G-E-S
We flying the first class
Up in the sky
Poppin' M.smeg.
Livin' my life
In the fast lane
And I won't change
For the glamorous, oh infecting bacteria, bacteria."
|
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Jan 13, 2016 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina |
| Sequencer Used | Illumina MiSeq |
| Read Type | Single-end reads |
| Read Length | 150 bp |
| Approximate Shotgun Coverage | 1276 |
| Genome length (bp) | 68716 |
| Character of genome ends | Circularly Permuted |
| GC Content | 66.3% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | B |
| Subcluster | B1 |
| Cluster Life Cycle | Lytic |
| Other Cluster Members |
|
| Annotating Institution | Unknown or unassigned |
| Annotation Status | In GenBank |
| Plaque Notes | Plaque shape noted as circular, size is 3.5mm on average , and turbidity has been consistently slightly turbid.
|
| Morphotype | Siphoviridae |
| Number of Genes | 103 |
| Number of tRNAs | 0 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Submitted Annotation in DNA Master Format | Download |
| Submitted Minimal DNA Master File | Download |
| Author List | Download |
| Annotation Cover Sheet | Download |
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | MG757165 |
| Refseq Number | None yet |
| Archiving Info |
| Archiving status |
Archived |
| Pitt Freezer Box# |
25 |
| Pitt Freezer Box Grid# |
F12 |
| Available Files |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| EM Picture | Download |
| Final DNAMaster File | Download |
| GenBank File for Phamerator | Download |
