| Detailed Information for Phage PhrodoBaggins |
| Discovery Information |
| Isolation Host | Mycobacterium smegmatis mc²155 |
| Found By | Sravya Koduri |
| Year Found | 2015 |
| Location Found | Philadelphia, PA USA |
| Finding Institution | Drexel University |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Unknown |
| Isolation Temperature | Not entered |
| GPS Coordinates | 39.95675 N, 75.189806 W Map |
| Discovery Notes | Date of collection: 09/21/2015
Time of collection: 5:15 pm
Depth of soil dug: 2.5 inches
Approximate Temperature at time of collection: 70ºF
General Location: Computing and Informatics Garden
Approximate Altitude: 25.80 m
Sample Site Description: The sample site area is a secluded area near flowers and shrubs. It is located near the roots of a plant and the area is damp and well shaded.
|
| Naming Notes | This phage is named after one of the main protagonists in the Lord of the Rings books. |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Jan 13, 2016 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina Sequencing |
| Approximate Shotgun Coverage | 1334 |
| Genome length (bp) | 68873 |
| Character of genome ends | Circularly Permuted |
| GC Content | 66.4% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | B |
| Subcluster | B1 |
| Cluster Life Cycle | Lytic |
| Other Cluster Members |
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| Annotating Institution | Drexel University |
| Annotation Status | In GenBank |
| Plaque Notes | Throughout this experiment, the phages were incubated in the M. smegmatis culture for at least 24 hours but up to 48 hours. Plaques formed by this phage in M. smegmatis culture are consistently round and clear and approximately 3-4 mm in diameter. |
| Morphotype | Siphoviridae |
| Number of Genes | 105 |
| Number of tRNAs | 0 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
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| Submitted Annotation in DNA Master Format | Download |
| Submitted Minimal DNA Master File | Download |
| Author List | Download |
| Annotation Cover Sheet | Download |
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | MH399786 |
| Refseq Number | None yet |
| Archiving Info |
| Archiving status |
Archived |
| Pitt Freezer Box# |
25 |
| Pitt Freezer Box Grid# |
G4 |
| Available Files |
| Plaque Picture | Download |
| GenBank File for Phamerator | Download |
