| Detailed Information for Phage Phunky |
| Discovery Information |
| Isolation Host | Mycobacterium smegmatis mc²155 |
| Found By | Tru Walor |
| Year Found | 2016 |
| Location Found | Philadelphia, PA USA |
| Finding Institution | Drexel University |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | Not entered |
| GPS Coordinates | 39.955007 N, 75.189351 W Map |
| Discovery Notes | I collected my soil on 9/27/16 at 11:57 am. I dug 2 inches into the soil. It was 69 degrees Fahrenheit and there was a light sprinkling rain. It was cold but slightly humid outside. I found my soil by the subway station at Drexel University. |
| Naming Notes | I named this phage Phunky because the area where I collected my soil around smelled funky. |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Dec 19, 2016 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina |
| Sequencer Used | Illumina MiSeq |
| Read Type | Single-end reads |
| Read Length | 150 bp |
| Approximate Shotgun Coverage | 1100 |
| Genome length (bp) | 68375 |
| Character of genome ends | Circularly Permuted |
| GC Content | 66.5% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | B |
| Subcluster | B1 |
| Cluster Life Cycle | Lytic |
| Other Cluster Members |
|
| Annotating Institution | Unknown or unassigned |
| Annotation Status | In GenBank |
| Morphotype | Siphoviridae |
| Number of Genes | 99 |
| Number of tRNAs | 0 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Submitted Annotation in DNA Master Format | Download |
| Submitted Minimal DNA Master File | Download |
| Author List | Download |
| Annotation Cover Sheet | Download |
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | MF919528 |
| Refseq Number | None yet |
| Archiving Info |
| Archiving status |
Archived |
| SEA Lysate Titer |
9 x 10^10 |
| Date of SEA Lysate Titering |
Nov 7, 2016 |
| Pitt Freezer Box# |
38 |
| Pitt Freezer Box Grid# |
F2 |
| Available Files |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| EM Picture | Download |
| Final DNAMaster File | Download |
| GenBank File for Phamerator | Download |
