| Detailed Information for Phage Pipsqueak |
| Discovery Information |
| Isolation Host | Mycobacterium smegmatis mc²155 |
| Found By | Champlin Park H.S. Microbiology Club |
| Year Found | 2011 |
| Location Found | Maple Grove, MN USA |
| Finding Institution | Champlin Park High School |
| Program | Phage Hunters Integrating Research and Education |
| From enriched soil sample? | Unknown |
| Isolation Temperature | Not entered |
| GPS Coordinates | 45.122386 N, 93.443223 W Map |
| Discovery Notes | The sample was from the compost at the Lynde Garden Center and Nursery in Maple Grove, MN. It was obtained in early October, 2011. |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Oct 12, 2012 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Ion Torrent |
| Approximate Shotgun Coverage | 75 |
| Genome length (bp) | 68328 |
| Character of genome ends | Circularly Permuted |
| GC Content | 66.5% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | B |
| Subcluster | B1 |
| Cluster Life Cycle | Lytic |
| Other Cluster Members |
|
| Annotating Institution | Unknown or unassigned |
| Annotation Status | In GenBank |
| Plaque Notes | After 24 hours the plaque diameter size was between .5 and .75 mm. After 48 hours the plaques were about 1.5 mm in diameter. They were clear in the center with halos around the edges. |
| Morphotype | Siphoviridae |
| Number of Genes | 99 |
| Number of tRNAs | 0 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | KP027208 |
| Refseq Number | None yet |
| Published in Paper | Pope, Bowman, Russell, et al 2015, eLIFE: Whole genome comparison of a large collection of mycobacteriophages reveals a continuum of phage genetic diversity |
| Archiving Info |
| Archiving status |
Archived |
| Pitt Freezer Box# |
2 |
| Pitt Freezer Box Grid# |
F12 |
| Available Files |
| Plaque Picture | Download |
| Final DNAMaster File | Download |
