| Detailed Information for Phage Plmatters |
| Discovery Information |
| Isolation Host | Mycobacterium smegmatis mc²155 |
| Duplicates of this Phage | JulesWinnfield, Raju |
| Found By | Taylor Burroughs |
| Year Found | 2016 |
| Location Found | Washington, DC USA |
| Finding Institution | Howard University |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | No |
| Isolation Temperature | Not entered |
| GPS Coordinates | 38.9256 N, 77.0212 W Map |
| Discovery Notes | This sample was found inside the Greene Stadium at Howard University. It was taken from soil located on the outer edges of the running track, surrounded by dirt and grass. There is light traffic, along with buildings around the area. The soil was collected from 0.5 cm within the dirt. The weather outside was recorded to be 82 degrees Fahrenheit. |
| Naming Notes | I am a firm believer in standing up for people who are underrepresented in society. I applied this to a phrase "Plaque Lives Matter". This is what "Plmatters" stands for. |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Jan 17, 2017 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina Sequencing |
| Approximate Shotgun Coverage | 1915 |
| Genome length (bp) | 68318 |
| Character of genome ends | Circularly Permuted |
| GC Content | 66.5% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | B |
| Subcluster | B1 |
| Cluster Life Cycle | Lytic |
| Other Cluster Members |
|
| Annotating Institution | University of Pittsburgh |
| Annotation Status | In GenBank |
| Plaque Notes | The phages are temperate, and ususally range from about 1-2 mm. |
| Morphotype | Siphoviridae |
| Number of Genes | 99 |
| Number of tRNAs | 0 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Submitted Minimal DNA Master File | Download |
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | MK279871 |
| Refseq Number | None yet |
| Archiving Info |
| Archiving status |
Archived |
| SEA Lysate Titer |
1.4 x 10^11 |
| Date of SEA Lysate Titering |
Oct 17, 2016 |
| Pitt Freezer Box# |
44 |
| Pitt Freezer Box Grid# |
C6 |
| Available Files |
| Final DNAMaster File | Download |
| GenBank File for Phamerator | Download |