| Detailed Information for Phage SDcharge11 |
| Discovery Information |
| Isolation Host | Mycobacterium smegmatis mc²155 |
| Found By | Raymond Totah |
| Year Found | 2011 |
| Location Found | Los Angeles, CA USA |
| Finding Institution | Loyola Marymount University |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | Not entered |
| GPS Coordinates | 33.97 N, 118.418055 W Map |
| Discovery Notes | Depth: 1 - 2 inches deep
Temperature: 68 F
Soil was moist
Environment: tree, small bushes, shady |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Feb 26, 2012 |
| Sequencing Facility | Virginia Commonwealth University Nucleic Acids Research Facilities |
| Shotgun Sequencing Method | 454 |
| Sequencer Used | 454 Genome Sequencer FLX |
| Approximate Shotgun Coverage | 140 |
| Genome length (bp) | 67702 |
| Character of genome ends | Circularly Permuted |
| GC Content | 66.5% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | B |
| Subcluster | B1 |
| Cluster Life Cycle | Lytic |
| Other Cluster Members |
|
| Annotating Institution | Unknown or unassigned |
| Annotation Status | In GenBank |
| Plaque Notes | Size: 2 - 3 mm
Lytic
Circular shaped, smooth edges
Incubated at 37 C (for approx. 24 hours each time) |
| Morphotype | Siphoviridae |
| Number of Genes | 100 |
| Number of tRNAs | 0 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | KC661274 |
| Refseq Number | NC_021303 |
| Published in Paper | Pope, Bowman, Russell, et al 2015, eLIFE: Whole genome comparison of a large collection of mycobacteriophages reveals a continuum of phage genetic diversity |
| Archiving Info |
| Archiving status |
Archived |
| SEA Designator |
2011LOMAsdcharge11RT |
| SEA Lysate Titer |
3.10e10 |
| Date of SEA Lysate Titering |
Oct 25, 2011 |
| Pitt Freezer Box# |
25 |
| Pitt Freezer Box Grid# |
71 |
| Available Files |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| EM Picture | Download |
