| Discovery Information |
| Isolation Host | Mycobacterium smegmatis mc²155 |
| Found By | Mariam Dar |
| Year Found | 2016 |
| Location Found | North Brunswick, NJ USA |
| Finding Institution | University of the Sciences (now St. Joseph's Univ) |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | Not entered |
| GPS Coordinates | 40.445858 N, 74.52067 W Map |
| Discovery Notes | The moist soil sample was taken 2.5 cm deep from the organic vegetable patch in my backyard, where tomato plants are grown. |
| Naming Notes | The local park in North Brunswick is called Sabella Park, where I searched for soil samples this summer. As a child, I remember loving the swings and playing soccer at this park, so I named my phage "sabella". |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Feb 16, 2017 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina |
| Approximate Shotgun Coverage | 164 |
| Genome length (bp) | 67304 |
| Character of genome ends | Circularly Permuted |
| GC Content | 68.9% |
| Sequencing Notes | This genome was sequenced as part of a DOGEMS sample. |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | B |
| Subcluster | B2 |
| Cluster Life Cycle | Lytic |
| Other Cluster Members |
|
| Annotating Institution | University of the Sciences (now St. Joseph's Univ) |
| Annotation Status | In GenBank |
| Plaque Notes | Sabella has two different morphologies: some of the plaques are 0.1 cm in diameter, while others are 0.15 cm in diameter. This notable difference was tested multiple times, so the phage population of this lytic phage is consistent and contains two morphologies. All the plaques are clear and round with smooth edges. |
| Morphotype | Siphoviridae |
| Number of Genes | 92 |
| Number of tRNAs | 0 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Submitted Annotation in DNA Master Format | Download |
| Submitted Minimal DNA Master File | Download |
| Author List | Download |
| Annotation Cover Sheet | Download |
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | MH590591 |
| Refseq Number | None yet |
| Archiving Info |
| Archiving status |
Archived |
| SEA Lysate Titer |
8.7*10^10 pfu/mL |
| Date of SEA Lysate Titering |
Oct 4, 2016 |
| Pitt Freezer Box# |
34 |
| Pitt Freezer Box Grid# |
F9 |
| Available Files |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| EM Picture | Download |
| GenBank File for Phamerator | Download |
