| Detailed Information for Phage Schadenfreude |
| Discovery Information |
| Isolation Host | Mycobacterium smegmatis mc²155 |
| Found By | Olivia Urso |
| Year Found | 2015 |
| Location Found | Bowling Green, KY USA |
| Finding Institution | Western Kentucky University |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | No |
| Isolation Temperature | 37°C |
| GPS Coordinates | 37.001511 N, 86.429974 W Map |
| Discovery Notes | My soil sample that contained my phage came from the bank of the Barren River underneath a walking bridge. |
| Naming Notes | The word "schadenfreude" means pleasure derived from another person's misfortune. In a sense, phages do just that: thrive in another organisms (bacterial cells) misfortune, or death. |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Mar 1, 2016 |
| Shotgun Sequencing Method | Illumina |
| Approximate Shotgun Coverage | 252 |
| Genome length (bp) | 68591 |
| Character of genome ends | Circularly Permuted |
| GC Content | 66.5% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | B |
| Subcluster | B1 |
| Cluster Life Cycle | Lytic |
| Other Cluster Members |
|
| Annotating Institution | Western Kentucky University |
| Annotation Status | In GenBank |
| Plaque Notes | My plaques were approximately .4 mm in diameter, very circular, and extremely clear. No turbidity was ever observed when streaking my plates. |
| Morphotype | Siphoviridae |
| Number of Genes | 102 |
| Number of tRNAs | 0 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Submitted Minimal DNA Master File | Download |
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | MH926060 |
| Refseq Number | None yet |
| Archiving Info |
| Archiving status |
Archived |
| SEA Designator |
2015WEKUSchadenfreudeOU |
| SEA Lysate Titer |
8.5 x 10^11 |
| Date of SEA Lysate Titering |
Oct 16, 2015 |
| Pitt Freezer Box# |
27 |
| Pitt Freezer Box Grid# |
F8 |
| Available Files |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| EM Picture | Download |
| GenBank File for Phamerator | Download |
