The Actinobacteriophage Database

Mycobacterium phage Scrick

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Detailed Information for Phage Scrick
Discovery Information
Isolation Host	Mycobacterium smegmatis mc²155
Found By	Jacqui Kokinda
Year Found	2022
Location Found	Nesquehoning, PA United States
Finding Institution	Saint Joseph's University
Program	Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?	Yes
Isolation Temperature	37°C
GPS Coordinates	40.86731 N, 75.810958 W Map
Discovery Notes	The soil was collected on 1/14/22 at 5:46 pm. The sample was collected out of the Nesquehoning Creek. The access point across from CVS was used (CVS address 20 E Locust St, Nesquehoning, PA 18240). Description of the site: Across from the CVS, there is a path to get down to the creek. Follow the path, and there is a bench at the end of the path right before the bank of the Creek. The soil was extracted 3 ft in front of the center of the bench. The soil was very dry, barely any moisture. It snowed on 1/7/22 and there was a storm with mostly ice and rain on 1/9/22. The soil was extracted 33 cm down.
Naming Notes	The bacteriophage was extracted right next to the Nesquehoning Creek. Years ago, the sewage from the town ran into the creek, or "crick" as the locals pronounce it. The creek to this day is referred to as the "Sewage Crick," so the bacteriophage name is shortened to Scrick.
Sequencing Information
Sequencing Complete?	Yes
Date Sequencing Completed	Jul 12, 2022
Sequencing Facility	Pittsburgh Bacteriophage Institute
Shotgun Sequencing Method	Illumina
Approximate Shotgun Coverage	476
Genome length (bp)	68701
Character of genome ends	Circularly Permuted
GC Content	66.4%
Fasta file available?	Yes: Download fasta file
Characterization
Cluster	B
Subcluster	B1
Cluster Life Cycle	Lytic
Other Cluster Members	Click to View AbsoluteMadLad (B1) ABU (B1) Adriana (B1) Aelin (B1) AltPhacts (B1) Altwerkus (B1) Anderson (B1) Andre (B1) Antonia (B1) Apizium (B1) Ashraf (B1) Badfish (B1) Banjo (B1) Basato (B1) BatteryCK (B1) Beaglebox (B1) Bishoperium (B1) BlackStallion (B1) BlueHusk (B1) Bluephacebaby (B1) Boehler (B1) Brilliant (B1) Buckeye (B1) Burr (B1) CamL (B1) CampRoach (B1) Cannibal (B1) Carthage (B1) Chaelin (B1) Chah (B1) Charles1 (B1) CharlieGBrown (B1) CheetO (B1) Cher (B1) Childish (B1) Chorkpop (B1) Chunky (B1) Chute (B1) Cobra (B1) Colbert (B1) Coletti (B1) Cornobble (B1) Cosmolli16 (B1) Craff (B1) Crownjwl (B1) DaddyDaniels (B1) Daffy (B1) Daka (B1) Dati (B1) DelRivs (B1) Derpp (B1) Dice (B1) Dingo (B1) Dione (B1) DirtJuice (B1) Doddsville (B1) DoesntMatter (B1) DonSanchon (B1) DuchessDung (B1) Duggie (B1) Durga (B1) Emiris (B1) EmpTee (B1) Eremos (B1) Etaye (B1) Eugenia (B1) Fang (B1) FluffyNinja (B1) Fozzie (B1) Frankicide (B1) Freya (B1) FriarPreacher (B1) Fringe (B1) FugateOSU (B1) Gareth (B1) GeneCoco (B1) Giraffe (B1) Gophee (B1) Grand2040 (B1) Gwilliam (B1) Gyarad (B1) Haimas (B1) Haleema (B1) Hamish (B1) Hartsy (B1) Harvey (B1) Held (B1) HenryJackson (B1) Hertubise (B1) Hetaeria (B1) HighStump (B1) Hocus (B1) Horchata (B1) HSavage (B1) ImtiyazSitla (B1) Inchworm (B1) Inverness (B1) Iridoclysis (B1) IsaacEli (B1) JacAttac (B1) JakeO (B1) JangoPhett (B1) JDog (B1) Jillium (B1) Jiminy (B1) Jiraiya (B1) Kahve (B1) Kailash (B1) Katniss (B1) Keitherie (B1) Kikipoo (B1) Kimbrough (B1) KingTut (B1) KingVeVeVe (B1) KlimbOn (B1) Kloppinator (B1) KLucky39 (B1) Kwadwo (B1) Kwksand96 (B1) Labeouficaum (B1) LasagnaCat (B1) Lasso (B1) LeeLot (B1) Lego3393 (B1) Legolas (B1) LeiMonet (B1) LemonSlice (B1) Longacauda (B1) Lopsy (B1) LostAndPhound (B1) LuckyMarjie (B1) Lulumae (B1) Lulwa (B1) Lumine (B1) Mag7 (B1) Magic8 (B1) Mana (B1) Manad (B1) Maru (B1) Maskar (B1) Matalotodo (B1) Mcshane (B1) Mecca (B1) Megamind20 (B1) Megatron (B1) Melc17 (B1) MelsMeow (B1) Mesh1 (B1) MichaelPhcott (B1) Mikota (B1) MiniBoss (B1) MitKao (B1) Morgushi (B1) Morty (B1) Mosaic (B1) MRabcd (B1) MrPhizzler (B1) Mulan (B1) Murdoc (B1) Mutante (B1) Nacho (B1) Nathan (B1) Newman (B1) Nicole21 (B1) Numberten (B1) Nyala (B1) Olak (B1) Oline (B1) Olive (B1) OliverWalter (B1) Omniscient (B1) Oosterbaan (B1) Orfeu (B1) Orion (B1) Orwigg (B1) OSmaximus (B1) Pacifista (B1) PG1 (B1) Phamished (B1) Phareon (B1) PhatCats2014 (B1) PhatLouie (B1) PhenghisKhan (B1) Pherdinand (B1) Phergie (B1) Phipps (B1) Phleuron (B1) PhrankReynolds (B1) PhrodoBaggins (B1) Phunky (B1) Piglet (B1) PinheadLarry (B1) Pinkman (B1) Pipsqueak (B1) Placalicious (B1) Plmatters (B1) Podrick (B1) Pops (B1) Potter (B1) Prickles (B1) ProfessorX (B1) Puhltonio (B1) QueenBeane (B1) Quisquiliae (B1) RedMaple (B1) Ricotta (B1) Riggan (B1) Rimu (B1) Robyn (B1) Roliet (B1) Roscoe (B1) Roy17 (B1) Samaymay (B1) SassyCat97 (B1) Schadenfreude (B1) Schueller (B1) Scoot17C (B1) Scrick (B1) SDcharge11 (B1) Selr12 (B1) Serendipity (B1) Serpentine (B1) Sheila (B1) ShiVal (B1) Sigman (B1) SilverBell (B1) Simielle (B1) Skippy (B1) Slatt (B1) Soile (B1) Solosis (B1) Sophia (B1) Soto (B1) Spartan300 (B1) Squid (B1) Squiggle (B1) Struggle (B1) Suffolk (B1) Surely (B1) Swiphy (B1) Swish (B1) TallGrassMM (B1) Telesworld (B1) Thora (B1) ThreeOh3D2 (B1) Thunderbird (B1) Timmi (B1) TomBombadil (B1) Tomlarah (B1) Toni (B1) Tooj (B1) True (B1) Trypo (B1) TyrionL (B1) UAch1 (B1) UncleHowie (B1) Usavi (B1) Vaishali24 (B1) Valjean (B1) Vaticameos (B1) Veritas (B1) Virapocalypse (B1) Virgeve (B1) Vista (B1) Vivaldi (B1) Vortex (B1) Wallhey (B1) Waterdiva (B1) Weher20 (B1) Windsor (B1) Xavier (B1) Yoshand (B1) YouGoGlencoco (B1) Zaider (B1) Zelda (B1) Zonia (B1) Imvubu (B10) Indlovu (B11) LilMcDreamy (B12) BirdsNest (B13) Hashim76 (B13) Zenteno07 (B13) Allegro (B2) Arbiter (B2) Ares (B2) Bananafish (B2) Blocker23 (B2) Boyle (B2) Brownie5 (B2) Calamitous (B2) Coffee (B2) Eaglehorse (B2) Faze9 (B2) FrenchFry (B2) Glass (B2) Godines (B2) Hedgerow (B2) Holeinone (B2) ItsyBitsy1 (B2) Kaleb (B2) Kheth (B2) Lars (B2) Laurie (B2) Lephleur (B2) LizLemon (B2) MasterPo (B2) Opia (B2) Phantasmagoria (B2) Qyrzula (B2) Rhinoforte (B2) Rosebush (B2) Sabella (B2) TA17A (B2) Tinciduntsolum (B2) Tres (B2) Vic9 (B2) West99 (B2) Abinghost (B3) Akoma (B3) Athena (B3) Audrey (B3) Baloo (B3) Ben11 (B3) Bernardo (B3) Briakila (B3) Casbah (B3) ChaChing (B3) Chandler (B3) Compostia (B3) Corofin (B3) Daisy (B3) Deenasa (B3) Devonte (B3) Gadjet (B3) Gervas (B3) GlenHope (B3) Halfpint (B3) HarveySr (B3) Heathcliff (B3) Jackstina (B3) Kamiyu (B3) Kronus (B3) LestyG (B3) Marley1013 (B3) MmasiCarm (B3) Mortcellus (B3) Morty007 (B3) Neos5 (B3) Nozo (B3) Obutu (B3) OrangeOswald (B3) Phaedrus (B3) Phayeta (B3) Philly (B3) Phlyer (B3) Pipefish (B3) RagingRooster (B3) Rita1961 (B3) RomaT (B3) SlippinJimmy (B3) SynergyX (B3) Tydolla (B3) Yahalom (B3) Yinz (B3) Ahwei (B4) AlanGrant (B4) Antihero (B4) Apex (B4) Austelle (B4) BobbyK (B4) BrownCNA (B4) ChrisnMich (B4) Cooper (B4) Epah (B4) Fanaw (B4) Fortunato (B4) Frederick (B4) GinPorsche (B4) Hally898 (B4) Hangman (B4) Heath (B4) Hydro (B4) JaguarMi (B4) JAMaL (B4) Lambano (B4) Lolalove (B4) Magpie (B4) Mithril (B4) Mudslide (B4) Nanao (B4) Nigel (B4) Poster (B4) Prince (B4) QueenPie (B4) RawrgerThat (B4) Stinger (B4) Vincenzo (B4) VioletZ (B4) Waleliano (B4) Xincheng (B4) Zemanar (B4) Acadian (B5) Baee (B5) DigitDog (B5) Donny (B5) EastView9101 (B5) KarasumaRum (B5) Mysterious (B5) Nangang (B5) Nehemiah (B5) Phelemich (B5) Reprobate (B5) Rich (B5) Serendipitous (B5) Shenyu (B5) SirJeffery (B5) StinkyVeggy (B5) Suigeneris (B5) 39HC (B6) 40BC (B6) Hosp (B6) Jolie1 (B6) KayaCho (B6) Saguaro (B7) Thonko (B8) CRB2 (B9) Funsized (B9) Knocker (B9) PenguinLover67 (B9) Quesadilla (B9) Rinkes (B9)
Annotating Institution	Saint Joseph's University
Annotation Status	In GenBank
Plaque Notes	The average plaque diameter for Scrick is 0.83 mm. The plagues are usually very clear and emerge after 24 hours in a 37°C incubator.
Morphotype	Siphoviridae
Number of Genes	103
Number of tRNAs	0
Number of tmRNAs	0
Has been Phamerated?	Yes
Gene List	Click to View Scrick_1 (adenylate kinase) Scrick_2 (terminase) Scrick_3 Scrick_4 Scrick_5 Scrick_6 (RuvC-like resolvase) Scrick_7 Scrick_8 (portal protein) Scrick_9 (capsid maturation protease) Scrick_10 Scrick_11 (major capsid hexamer protein) Scrick_12 (major capsid pentamer protein) Scrick_13 Scrick_14 Scrick_15 Scrick_16 Scrick_17 (major tail protein) Scrick_18 Scrick_19 Scrick_20 Scrick_21 Scrick_22 (head-to-tail adaptor) Scrick_23 Scrick_24 Scrick_25 Scrick_26 Scrick_27 Scrick_28 (tape measure protein) Scrick_29 (minor tail protein) Scrick_30 (minor tail protein) Scrick_31 (minor tail protein) Scrick_32 (minor tail protein) Scrick_33 (minor tail protein) Scrick_34 Scrick_35 Scrick_36 Scrick_37 Scrick_38 Scrick_39 Scrick_40 Scrick_41 Scrick_42 Scrick_43 Scrick_44 Scrick_45 (helix-turn-helix DNA binding domain protein) Scrick_46 (helix-turn-helix DNA binding domain protein) Scrick_47 Scrick_48 (lysin A) Scrick_49 (lysin B) Scrick_50 Scrick_51 Scrick_52 (DNA helicase) Scrick_53 Scrick_54 Scrick_55 Scrick_56 Scrick_57 Scrick_58 Scrick_59 (DNA primase/helicase) Scrick_60 Scrick_61 (DNA polymerase I) Scrick_62 Scrick_63 Scrick_64 Scrick_65 Scrick_66 Scrick_67 (ribbon-helix-helix DNA binding domain protein) Scrick_68 Scrick_69 (helix-turn-helix DNA binding domain protein) Scrick_70 Scrick_71 Scrick_72 Scrick_73 Scrick_74 Scrick_75 Scrick_76 Scrick_77 Scrick_78 Scrick_79 Scrick_80 Scrick_81 Scrick_82 Scrick_83 (HNH endonuclease) Scrick_84 Scrick_85 Scrick_86 Scrick_87 Scrick_88 Scrick_89 Scrick_90 Scrick_91 Scrick_92 Scrick_93 Scrick_94 Scrick_95 Scrick_96 Scrick_97 Scrick_98 Scrick_99 Scrick_100 Scrick_101 Scrick_102 Scrick_103
Submitted Annotation in DNA Master Format	Download
Submitted Minimal DNA Master File	Download
Author List	Download
Annotation Cover Sheet	Download
Publication Info
Uploaded to GenBank?	Yes
GenBank Accession	OR521069
Refseq Number	None yet
Archiving Info
Archiving status	Archived
Pitt Freezer Box#	146
Pitt Freezer Box Grid#	B11
Available Files
Plaque Picture	Download
Restriction Digest Picture	Download
EM Picture	Download
GenBank File for Phamerator	Download

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