Mycobacterium phage Serendipitous
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| Detailed Information for Phage Serendipitous | |
| Discovery Information | |
| Isolation Host | Mycobacterium smegmatis mc²155 |
| Found By | Lindsey Farris |
| Year Found | 2015 |
| Location Found | High Shoals, NC USA |
| Finding Institution | Western Carolina University |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | No |
| Isolation Temperature | Not entered |
| GPS Coordinates | 35.4026 N, 81.2023 W Map |
| Discovery Notes | This phage was found in a sample that was collected from a home-built compost pile. The soil was from the bottom of the compost pile, about two feet in the compost box. |
| Naming Notes | The name for this phage was chosen because the word 'serendipitous' means by luck or chance, which is what the entire process of finding and isolating phage has been. |
| Sequencing Information | |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Dec 9, 2015 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina |
| Sequencer Used | Illumina MiSeq |
| Read Type | Single-end reads |
| Read Length | 150 bp |
| Approximate Shotgun Coverage | 999 |
| Genome length (bp) | 69867 |
| Character of genome ends | Circularly Permuted |
| GC Content | 68.0% |
| Fasta file available? | Yes: Download fasta file |
| Characterization | |
| Cluster | B |
| Subcluster | B5 |
| Cluster Life Cycle | Lytic |
| Other Cluster Members | |
| Annotating Institution | Western Carolina University |
| Annotation Status | In GenBank |
| Plaque Notes | The plaques that this phages makes are extremely small and can be difficult to see. |
| Morphotype | Siphoviridae |
| Number of Genes | 98 |
| Number of tRNAs | 0 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List | |
| Submitted Annotation in DNA Master Format | Download |
| Submitted Minimal DNA Master File | Download |
| Author List | Download |
| Annotation Cover Sheet | Download |
| Publication Info | |
| Uploaded to GenBank? | Yes |
| GenBank Accession | MH727561 |
| Refseq Number | None yet |
| Archiving Info | |
| Archiving status | Archived |
| SEA Lysate Titer | 2.816 x 10^9 |
| Date of SEA Lysate Titering | Nov 10, 2015 |
| Pitt Freezer Box# | 19 |
| Pitt Freezer Box Grid# | G11 |
| Available Files | |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| EM Picture | Download |
| GenBank File for Phamerator | Download |
