| Detailed Information for Phage Soto |
| Discovery Information |
| Isolation Host | Mycobacterium smegmatis mc²155 |
| Found By | Katie Fusco |
| Year Found | 2012 |
| Location Found | Los Angeles , CA USA |
| Finding Institution | Loyola Marymount University |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | Not entered |
| GPS Coordinates | 33.9700 N, 118.4180 W Map |
| Discovery Notes | Sunny day in Southern California approximately 75°F at 11:15 a.m. Located on the campus of Loyola Marymount University by the freshman dorms, specifically on the south side of Del Rey South. Soil sample was taken about 2 inches below the surface of the ground. Wood chips, bushes and trees surrounded the soil. The dirt was moist. |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Feb 5, 2013 |
| Sequencing Facility | Virginia Commonwealth University Nucleic Acids Research Facilities |
| Shotgun Sequencing Method | 454 |
| Sequencer Used | 454 Genome Sequencer FLX |
| Approximate Shotgun Coverage | 90 |
| Genome length (bp) | 67744 |
| Character of genome ends | Circularly Permuted |
| GC Content | 66.6% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | B |
| Subcluster | B1 |
| Cluster Life Cycle | Lytic |
| Other Cluster Members |
|
| Annotating Institution | Unknown or unassigned |
| Annotation Status | In GenBank |
| Plaque Notes | Lysogenic. Multiple changes of morphology through purification process. Approximately 0.2cm in diameter after about 24-48 hours |
| Morphotype | Siphoviridae |
| Number of Genes | 98 |
| Number of tRNAs | 0 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | KJ174157 |
| Refseq Number | NC_025438 |
| Published in Paper | Pope, Bowman, Russell, et al 2015, eLIFE: Whole genome comparison of a large collection of mycobacteriophages reveals a continuum of phage genetic diversity |
| Archiving Info |
| Archiving status |
Archived |
| SEA Designator |
2012LOMAsotoKF |
| SEA Lysate Titer |
4.50e10 |
| Date of SEA Lysate Titering |
Oct 24, 2012 |
| Pitt Freezer Box# |
36 |
| Pitt Freezer Box Grid# |
74 |
| Available Files |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| EM Picture | Download |
