| Detailed Information for Phage Squiggle |
| Discovery Information |
| Isolation Host | Mycobacterium smegmatis mc²155 |
| Found By | Caleb Curry |
| Year Found | 2016 |
| Location Found | Bowling Green, KY USA |
| Finding Institution | Western Kentucky University |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | 30°C |
| GPS Coordinates | 36.98112 N, 86.456531 W Map |
| Discovery Notes | This phage was found in the compost pile at the Western Kentucky University Office of Sustainability. The soil had a low/medium moisture content, and was full of organic matter. I collected the sample from the surface of the compost pile on September 9, 2016 at 6:15pm CST. |
| Naming Notes | My phage name comes from the resemblance between phage tails and squiggles. |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Dec 30, 2016 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina |
| Sequencer Used | Illumina MiSeq |
| Read Type | Single-end reads |
| Read Length | 150 bp |
| Approximate Shotgun Coverage | 1578 |
| Genome length (bp) | 68325 |
| Character of genome ends | Circularly Permuted |
| GC Content | 66.5% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | B |
| Subcluster | B1 |
| Cluster Life Cycle | Lytic |
| Other Cluster Members |
|
| Annotating Institution | Western Kentucky University |
| Annotation Status | In GenBank |
| Plaque Notes | My phage created clear plaques, with a slight ring of turbidity. The plaques were approximately 1-2mm in diameter after 48 hours of incubation at 30 degrees Celsius. |
| Morphotype | Siphoviridae |
| Number of Genes | 101 |
| Number of tRNAs | 0 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Submitted Minimal DNA Master File | Download |
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | MK112553 |
| Refseq Number | None yet |
| Archiving Info |
| Archiving status |
Archived |
| SEA Designator |
2016WEKUsquiggleCWC |
| SEA Lysate Titer |
7.2 x 10^10 pfu/mL |
| Date of SEA Lysate Titering |
Oct 13, 2016 |
| Pitt Freezer Box# |
37 |
| Pitt Freezer Box Grid# |
D6 |
| Available Files |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| EM Picture | Download |
| GenBank File for Phamerator | Download |
