| Detailed Information for Phage Thonko |
| Discovery Information |
| Isolation Host | Mycobacterium smegmatis mc²155 |
| Found By | Nomusa Christina Mzimela |
| Year Found | 2017 |
| Location Found | Empangeni, South Africa |
| Finding Institution | University of Kwazulu-Natal |
| Program | Mycobacterial Genetics Course, Durban, South Africa |
| From enriched soil sample? | Yes |
| Isolation Temperature | 37°C |
| GPS Coordinates | 28.8 S, 31.8 E Map |
| Discovery Notes | Thonko phage was discovered by Nomusa Christina Mzimela at a city of Empangeni in a place called Kwadlangezwa. It is a reddish brown soil collected from Mzimela residence yard.It was enriched with 7H9 media and incubated for 24 hours. |
| Naming Notes | Thonko is a name discovered fro my parents yard since I took the soil from home.I used the 3 letters of their names Thokozani(father) and Nkosingiphile(mother) |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Apr 18, 2018 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina |
| Sequencer Used | Illumina MiSeq |
| Read Type | Single-end reads |
| Read Length | 150 bp |
| Approximate Shotgun Coverage | 1574 |
| Genome length (bp) | 69471 |
| Character of genome ends | Circularly Permuted |
| GC Content | 68.6% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | B |
| Subcluster | B8 |
| Cluster Life Cycle | Lytic |
| Other Cluster Members |
|
| Annotating Institution | University of Kwazulu-Natal |
| Annotation Status | In GenBank |
| Plaque Notes | "Thonko" took a heart shape when growing then after 48 hours it grew and took a Y shape.It was transparent in colour. It has titre 5 x 10^11 |
| Morphotype | Siphoviridae |
| Number of Genes | 108 |
| Number of tRNAs | 0 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Submitted Minimal DNA Master File | Download |
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | MH632120 |
| Refseq Number | None yet |
| Archiving Info |
| Archiving status |
Not in Pitt Archives |
| Available Files |
| Plaque Picture | Download |
| EM Picture | Download |
| Final DNAMaster File | Download |
| GenBank File for Phamerator | Download |
