| Detailed Information for Phage Tres |
| Discovery Information |
| Isolation Host | Mycobacterium smegmatis mc²155 |
| Found By | Rachel Pallister |
| Year Found | 2014 |
| Location Found | Harrisonburg, VA USA |
| Finding Institution | James Madison University |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | Not entered |
| GPS Coordinates | 38.429196 N, 78.861398 W Map |
| Discovery Notes | This phage was found in the Arboretum at James Madison University near the birdhouse. |
| Naming Notes | I chose the name Tres for my phage because I found it on the third of September. |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Dec 19, 2014 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina |
| Sequencer Used | Illumina MiSeq |
| Read Type | Single-end reads |
| Read Length | 150 bp |
| Approximate Shotgun Coverage | 300 |
| Genome length (bp) | 67349 |
| Character of genome ends | Circularly Permuted |
| GC Content | 68.9% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | B |
| Subcluster | B2 |
| Cluster Life Cycle | Lytic |
| Lysogeny Notes | My plaques are turbid which leads me to believe my phage is temperate. It can do lytic or lysogenic cycle. |
| Other Cluster Members |
|
| Annotating Institution | Unknown or unassigned |
| Annotation Status | In GenBank |
| Plaque Notes | Plaques are turbid. |
| Morphotype | Siphoviridae |
| Number of Genes | 92 |
| Number of tRNAs | 0 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Submitted Annotation in DNA Master Format | Download |
| Submitted Minimal DNA Master File | Download |
| Author List | Download |
| Annotation Cover Sheet | Download |
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | KT365402 |
| Refseq Number | None yet |
| Archiving Info |
| Archiving status |
Archived |
| SEA Lysate Titer |
4.6 x 10^9 |
| Date of SEA Lysate Titering |
Oct 29, 2014 |
| Pitt Freezer Box# |
15 |
| Pitt Freezer Box Grid# |
B1 |
| Available Files |
| Plaque Picture | Download |