| Detailed Information for Phage Waterdiva |
| Discovery Information |
| Isolation Host | Mycobacterium smegmatis mc²155 |
| Found By | Ryan Benczik |
| Year Found | 2012 |
| Location Found | Fishers, IN USA |
| Finding Institution | Purdue University |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | No |
| Isolation Temperature | Not entered |
| GPS Coordinates | 39.928327 N, 85.970876 W Map |
| Discovery Notes | This phage was found in a recessed area of a stream. The sample was collected from still water right after it had rained. the sample was taken from the surface of the water right at the edge of the pond. The sample was collected at approximately 5:30p.m. on 9/4/12 |
| Naming Notes | We had allot of different ways that we could have named our bacteriophage. However, we decided on "waterdiva" because it was found from a pond water sample and we thought it could be a novel phage due to the exponential growth that it showed throughout the progression of its purification. |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Jun 13, 2013 |
| Sequencing Facility | Purdue Genomics Facility |
| Shotgun Sequencing Method | Illumina Sequencing |
| Approximate Shotgun Coverage | 394 |
| Genome length (bp) | 68866 |
| Character of genome ends | Circularly Permuted |
| GC Content | 66.5% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | B |
| Subcluster | B1 |
| Cluster Life Cycle | Lytic |
| Other Cluster Members |
|
| Annotating Institution | Purdue University |
| Annotation Status | In GenBank |
| Plaque Notes | the phage on the plate ate approximately a 4mm diameter of M.Smeg bacteria on the plate. Our first plate had 202 phage clusters.
Please also note: I have pictures from Electron Microscopy but they did not show up clearly and cannot begin characterization of my phage at this time. |
| Morphotype | Siphoviridae |
| Number of Genes | 103 |
| Number of tRNAs | 0 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Submitted Annotation in DNA Master Format | Download |
| Submitted Minimal DNA Master File | Download |
| Author List | Download |
| Annotation Cover Sheet | Download |
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | MH779516 |
| Refseq Number | None yet |
| Published in Paper | Pope, Bowman, Russell, et al 2015, eLIFE: Whole genome comparison of a large collection of mycobacteriophages reveals a continuum of phage genetic diversity |
| Archiving Info |
| Archiving status |
Archived |
| Pitt Freezer Box# |
39 |
| Pitt Freezer Box Grid# |
59 |
| Available Files |
| GenBank File for Phamerator | Download |