| Detailed Information for Phage Zonia |
| Discovery Information |
| Isolation Host | Mycobacterium smegmatis mc²155 |
| Found By | Abigail Brown |
| Year Found | 2013 |
| Location Found | St. Louis, MO USA |
| Finding Institution | Illinois Wesleyan University |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | Not entered |
| GPS Coordinates | 38.627222 N, 90.197778 W Map |
| Discovery Notes | Date: 9/2/13
Temperature: 82F
Location:The phage was found 3 cm deep in a garden containing a mixture of clay and soil. |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Jan 22, 2014 |
| Sequencing Facility | NC State Genomic Sciences Laboratory |
| Shotgun Sequencing Method | Illumina |
| Sequencer Used | Illumina MiSeq |
| Read Type | Single-end reads |
| Read Length | 150 bp |
| Approximate Shotgun Coverage | 4994 |
| Genome length (bp) | 69271 |
| Character of genome ends | Circularly Permuted |
| GC Content | 66.3% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | B |
| Subcluster | B1 |
| Cluster Life Cycle | Lytic |
| Other Cluster Members |
|
| Annotating Institution | Unknown or unassigned |
| Annotation Status | In GenBank |
| Plaque Notes | The plaques had to separate diameters of 2 cm and 5 cm. They are both clear and circular. |
| Morphotype | Siphoviridae |
| Number of Genes | 102 |
| Number of tRNAs | 0 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Submitted Annotation in DNA Master Format | Download |
| Author List | Download |
| Annotation Cover Sheet | Download |
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | KM363597 |
| Refseq Number | None yet |
| Published in Paper | Pope, Bowman, Russell, et al 2015, eLIFE: Whole genome comparison of a large collection of mycobacteriophages reveals a continuum of phage genetic diversity |
| Archiving Info |
| Archiving status |
Archived |
| SEA Lysate Titer |
5.9x10^10 |
| Pitt Freezer Box# |
4 |
| Pitt Freezer Box Grid# |
C9 |
| Available Files |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
