| Detailed Information for Phage BrownCNA |
| Discovery Information |
| Isolation Host | Mycobacterium smegmatis mc²155 |
| Former names | Browncna |
| Found By | Gerald Brown |
| Year Found | 2013 |
| Location Found | Atlanta, GA USA |
| Finding Institution | Morehouse College |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | 37°C |
| GPS Coordinates | 33.96269 N, 83.70152 W Map |
| Discovery Notes | In a flower bed, hot, surrounded by flowers. Sample site was hard, hot , and dry. It was 81 degrees. |
| Naming Notes | The name of this phage came from a hard long thinking process I had to do. |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Dec 16, 2013 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina |
| Sequencer Used | Illumina MiSeq |
| Read Type | Single-end reads |
| Read Length | 150 bp |
| Approximate Shotgun Coverage | 1433 |
| Genome length (bp) | 71214 |
| Character of genome ends | Circularly Permuted |
| GC Content | 68.9% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | B |
| Subcluster | B4 |
| Cluster Life Cycle | Lytic |
| Other Cluster Members |
|
| Annotating Institution | Unknown or unassigned |
| Annotation Status | In GenBank |
| Plaque Notes | Vertical diameter: 1.5 ml
Horizontal diameter: 1.6 ml |
| Morphotype | Siphoviridae |
| Number of Genes | 97 |
| Number of tRNAs | 0 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Submitted Annotation in DNA Master Format | Download |
| Submitted Minimal DNA Master File | Download |
| Author List | Download |
| Annotation Cover Sheet | Download |
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | KT270441 |
| Refseq Number | None yet |
| Published in Paper | Pope, Bowman, Russell, et al 2015, eLIFE: Whole genome comparison of a large collection of mycobacteriophages reveals a continuum of phage genetic diversity |
| Archiving Info |
| Archiving status |
Archived |
| SEA Lysate Titer |
3x10^8 |
| Date of SEA Lysate Titering |
Nov 4, 2013 |
| Pitt Freezer Box# |
11 |
| Pitt Freezer Box Grid# |
A12 |
| Available Files |
| Plaque Picture | Download |
