| Discovery Information |
| Isolation Host | Mycobacterium smegmatis mc²155 |
| Found By | Jordan Wonka |
| Year Found | 2022 |
| Location Found | Bowling Green, KY United States |
| Finding Institution | Western Kentucky University |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | No |
| Isolation Temperature | 30°C |
| GPS Coordinates | 37.013889 N, 86.457222 W Map |
| Discovery Notes | The water sample was collected from a shallow part of a creek (4-5 inches deep) which streamed off from the Barren river.
The temperature when collected was 34 degrees C.
The humidity was 62% and the surrounding soil was moist as it had recently rained.
The water sample was enriched and spot tests were used to determine if phage was present. An initial serial dilution was performed and plated. Purification of the water sample was done by picking a single plaque from the 10^-4 plate of the initial plated serial dilution and plating another serial dilution from the picked plaque. From the second serial dilution, a single plaque from the 10^-4 plate was picked, serially diluted, and plated. This process was repeated one more time. The webby plate from this final plated serial dilution was flooded with phage buffer and filtered to produce a high-titer lysate. |
| Naming Notes | I had just learned what the word cornobble meant, to slap someone with a fish, and I thought it was funny and fitting because the phage came from a river water sample. |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Apr 11, 2023 |
| Sequencing Facility | NC State Genomic Sciences Laboratory |
| Shotgun Sequencing Method | Illumina Sequencing |
| Approximate Shotgun Coverage | 5827 |
| Genome length (bp) | 68722 |
| Character of genome ends | Circularly Permuted |
| GC Content | 66.5% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | B |
| Subcluster | B1 |
| Cluster Life Cycle | Lytic |
| Lysogeny Notes | The plaques formed by Cornobble were clear, therefore, Cornobble is a lytic phage. |
| Other Cluster Members |
|
| Annotating Institution | Western Kentucky University |
| Annotation Status | In GenBank |
| Plaque Notes | The circular plaques range from 1mm to 4mm in diameter and are clear. The results were repeatable. |
| Morphotype | Siphoviridae |
| Number of Genes | 100 |
| Number of tRNAs | 0 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Submitted Minimal DNA Master File | Download |
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | PQ201078 |
| Refseq Number | None yet |
| Archiving Info |
| Archiving status |
Archived |
| SEA Lysate Titer |
9.3(10^10) |
| Pitt Freezer Box# |
157 |
| Pitt Freezer Box Grid# |
F6 |
| Available Files |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| EM Picture | Download |
| GenBank File for Phamerator | Download |
