| Detailed Information for Phage HSavage |
| Discovery Information |
| Isolation Host | Mycobacterium smegmatis mc²155 |
| Found By | Grace Ludlow and Hannah Dreesbach |
| Year Found | 2017 |
| Location Found | Helena, MT USA |
| Finding Institution | Montana Technological University |
| Program | Montana Tech Bringing Research Into the Classroom |
| From enriched soil sample? | Unknown |
| Isolation Temperature | 37°C |
| GPS Coordinates | 46.608443 N, 112.056423 W Map |
| Discovery Notes | Sample was taken from a compost pile in the Waukesha Park Community Garden in December of 2016. Sample had a high water content and was incubated for close to seven months with periodic tests. HSavage was isolated from this sample in June. |
| Naming Notes | The name of our phage was inspired by a fictional character that we invented for a creative writing assignment. |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Dec 19, 2017 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina |
| Sequencer Used | Illumina MiSeq |
| Read Type | Single-end reads |
| Read Length | 150 bp |
| Genome length (bp) | 67993 |
| Character of genome ends | Unknown |
| GC Content | 66.5% |
| Sequencing Notes | at position 30596 bp approximately 40% reads A to G
after position 67880 bp, consensus sequence contains string 12 c's, 30% of reads contain 11 or 13 G's |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | B |
| Subcluster | B1 |
| Cluster Life Cycle | Lytic |
| Other Cluster Members |
|
| Annotating Institution | Montana Technological University |
| Annotation Status | In GenBank |
| Plaque Notes | Small, circular, slightly cloudy. |
| Morphotype | Siphoviridae |
| Number of Genes | 99 |
| Number of tRNAs | 0 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Submitted Annotation in DNA Master Format | Download |
| Submitted Minimal DNA Master File | Download |
| Author List | Download |
| Annotation Cover Sheet | Download |
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | MH576954 |
| Refseq Number | None yet |
| Archiving Info |
| Archiving status |
Archived |
| Pitt Freezer Box# |
47 |
| Pitt Freezer Box Grid# |
B1 |
| Available Files |
| Plaque Picture | Download |
| EM Picture | Download |
| GenBank File for Phamerator | Download |
