| Detailed Information for Phage Heathcliff |
| Discovery Information |
| Isolation Host | Mycobacterium smegmatis mc²155 |
| Found By | Alexandra Cathcart |
| Year Found | 2011 |
| Location Found | Pittsburgh, PA USA |
| Finding Institution | University of Pittsburgh |
| Program | Phage Hunters Integrating Research and Education |
| From enriched soil sample? | No |
| Isolation Temperature | Not entered |
| GPS Coordinates | 40.444235 N, 79.95871 W Map |
| Discovery Notes | In a mulch bed; ~2 centimeters down
At the bottom of a slope
Soil moist; bed littered with trash |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Mar 12, 2013 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Ion Torrent Sequencing |
| Approximate Shotgun Coverage | 130 |
| Sanger Finishing Reactions | 6 |
| Genome length (bp) | 68628 |
| Character of genome ends | Circularly Permuted |
| GC Content | 67.5% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | B |
| Subcluster | B3 |
| Cluster Life Cycle | Lytic |
| Other Cluster Members |
|
| Annotating Institution | Unknown or unassigned |
| Annotation Status | In GenBank |
| Plaque Notes | Two different size plaques; one pinprick size and one about 1 mm in diameter
No turbidity; plaques very clear and round |
| Morphotype | Siphoviridae |
| Number of Genes | 103 |
| Number of tRNAs | 0 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | KJ194584 |
| Refseq Number | None yet |
| Published in Paper | Pope, Bowman, Russell, et al 2015, eLIFE: Whole genome comparison of a large collection of mycobacteriophages reveals a continuum of phage genetic diversity |
| Archiving Info |
| Archiving status |
Archived |
| Pitt Freezer Box# |
1 |
| Pitt Freezer Box Grid# |
H12 |
| Available Files |
| Plaque Picture | Download |
| EM Picture | Download |
| Final DNAMaster File | Download |
