Mycobacterium phage Heathcliff
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Detailed Information for Phage Heathcliff
Discovery Information
Isolation HostMycobacterium smegmatis mc²155
Found ByAlexandra Cathcart
Year Found2011
Location FoundPittsburgh, PA USA
Finding InstitutionUniversity of Pittsburgh
ProgramPhage Hunters Integrating Research and Education
From enriched soil sample?No
Isolation TemperatureNot entered
GPS Coordinates40.444235 N, 79.95871 W Map
Discovery NotesIn a mulch bed; ~2 centimeters down
At the bottom of a slope
Soil moist; bed littered with trash
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedMar 12, 2013
Sequencing FacilityPittsburgh Bacteriophage Institute
Shotgun Sequencing MethodIon Torrent
Approximate Shotgun Coverage130
Sanger Finishing Reactions6
Genome length (bp)68628
Character of genome endsCircularly Permuted
GC Content67.5%
Fasta file available?Yes: Download fasta file
Characterization
ClusterB
SubclusterB3
Cluster Life CycleLytic
Other Cluster Members
Annotating InstitutionUnknown or unassigned
Annotation StatusIn GenBank
Plaque NotesTwo different size plaques; one pinprick size and one about 1 mm in diameter
No turbidity; plaques very clear and round
MorphotypeSiphoviridae
Number of Genes103
Number of tRNAs0
Number of tmRNAs0
Has been Phamerated?Yes
Gene List
Publication Info
Uploaded to GenBank?Yes
GenBank AccessionKJ194584
Refseq NumberNone yet
Published in PaperPope, Bowman, Russell, et al 2015, eLIFE: Whole genome comparison of a large collection of mycobacteriophages reveals a continuum of phage genetic diversity
Archiving Info
Archiving status Archived
Pitt Freezer Box# 1
Pitt Freezer Box Grid# H12
Available Files
Plaque PictureDownload
EM PictureDownload
Final DNAMaster FileDownload