| Detailed Information for Phage JoyceMaria |
| Discovery Information |
| Isolation Host | Mycobacterium smegmatis mc²155 |
| Found By | Ally Ballard |
| Year Found | 2025 |
| Location Found | Joann, AR United States |
| Finding Institution | Ouachita Baptist University |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | No |
| Isolation Temperature | 31°C |
| GPS Coordinates | 34.13198 N, 92.93996 W Map |
| Discovery Notes | Found under a bridge walkway in muddy area. Hot, sunny, humid (61%) weather. Lots of trees and brush surrounding soil sample. |
| Naming Notes | The names of Ally Ballard and lab partner Emma Cokenour’s grandmothers. |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Jan 9, 2026 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina |
| Sequencer Used | Illumina NextSeq 1000 |
| Read Type | Single-end reads |
| Read Length | 100 bp |
| Approximate Shotgun Coverage | 1009 |
| Genome length (bp) | 71208 |
| Character of genome ends | Circularly Permuted |
| GC Content | 68.9% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | B |
| Subcluster | B4 |
| Cluster Life Cycle | Lytic |
| Other Cluster Members |
|
| Annotating Institution | Ouachita Baptist University |
| Annotation Status | Being Annotated (Expected completion by 5/1/2026) |
| Plaque Notes | Tiny, pinprick sized circular plaques. Plaques are consistently turbid. Later dilutions revealed two layers of plaques, the second one containing plaques with ≈0.5mm diameters and more irregular edges. These plaques are turbid as well. 1 type of phage isolated. |
| Morphotype | Siphoviridae |
| Number of Genes | 96 |
| Number of tRNAs | 0 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Publication Info |
| Uploaded to GenBank? | No |
| GenBank Accession | None yet |
| Refseq Number | None yet |
| Archiving Info |
| Archiving status |
Not in Pitt Archives |
| Available Files |
| Plaque Picture | Download |
