| Detailed Information for Phage Lasso |
| Discovery Information |
| Isolation Host | Mycobacterium smegmatis mc²155 |
| Found By | Barrett Miller |
| Year Found | 2013 |
| Location Found | Bethlehem, PA USA |
| Finding Institution | Lehigh University |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | Not entered |
| GPS Coordinates | 40.625 N, 75.378056 W Map |
| Discovery Notes | Lasso was isolated from the sediment at the bottom of a shallow stream bed. The sample was collected under approximately 5 inches of water and 2 inches of sediment. The temperature was 45 degrees Fahrenheit (7.2 degrees C). |
| Naming Notes | The name "Lasso" was inspired by the physical characteristics of the phage when viewed under the electron microscope. The long tail paired with the round shape of the head resembled a lasso. |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Oct 4, 2013 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Ion Torrent |
| Approximate Shotgun Coverage | 340 |
| Sanger Finishing Reactions | 2 |
| Genome length (bp) | 68576 |
| Character of genome ends | Circularly Permuted |
| GC Content | 66.4% |
| Sequencing Notes | This phage was inadvertently labeled as "Milly" during sequencing. |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | B |
| Subcluster | B1 |
| Cluster Life Cycle | Lytic |
| Other Cluster Members |
|
| Annotating Institution | Unknown or unassigned |
| Annotation Status | In GenBank |
| Plaque Notes | Plaques are small (1mm) and clear after 24 hours of incubation. |
| Morphotype | Siphoviridae |
| Number of Genes | 101 |
| Number of tRNAs | 0 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Submitted Annotation in DNA Master Format | Download |
| Author List | Download |
| Annotation Cover Sheet | Download |
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | KM408320 |
| Refseq Number | None yet |
| Published in Paper | Pope, Bowman, Russell, et al 2015, eLIFE: Whole genome comparison of a large collection of mycobacteriophages reveals a continuum of phage genetic diversity |
| Archiving Info |
| Archiving status |
Archived |
| SEA Designator |
2013LEHIlassoBDM |
| SEA Lysate Titer |
1.89x10^9 |
| Pitt Freezer Box# |
5 |
| Pitt Freezer Box Grid# |
F10 |
| Available Files |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| EM Picture | Download |
| Final DNAMaster File | Download |
