Mycobacterium phage Laurie
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Detailed Information for Phage Laurie
Discovery Information
Isolation HostMycobacterium smegmatis mc²155
Found ByTyler Williams
Year Found2013
Location FoundMilbridge, ME USA
Finding InstitutionUniversity of Maine, Machias
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?No
Isolation TemperatureNot entered
GPS Coordinates44.555822 N, 67.863971 W Map
Discovery NotesWhen I took the original soil sample from the environment I collected the following data:

The collected environmental soil sample was relatively dry to the touch. The surrounding land features near the site in which I collected my environmental soil sample are as follows:
- The sample was taken on a slope that lead to a road on the property. The road was primarily made from granite gravel. The road was approximately 15 feet away. Between the road and the site of the sample there appeared to be little foot traffic.

The entire farm was surrounded by forest. Approximately 5 feet away from the site where the environmental soil sample was taken were apple trees. Rotten apples covered a large portion of the ground. Their were no large bodies of water in close proximity to the Salty Dog Farm; the only body of water was a puddle approximately 14 feet away at the base of the slope along the side of the road. On the surface of the water was what appeared to be oil from a vehicle because whatever was floating on the waters surface was very colorful and looked metallic. I also noted that the land was sloped in a way so that if it were to rain, water would be channeled alongside the apple trees and down towards the road. The "channel" didn't appear to be man-made, instead it appeared that it was formed as a result of rain causing soil erosion.
I took the soil sample after I moved rotting apples, a decaying log, grass, and leaves off of the surface of the soil. I did this by using my bare hands and an all purpose shovel. The soil sample was taken at approximately 3 centimeters from the soils surface.
Approximately 2 feet from where I took the soil sample were a couple of snails. The snails shells were yellow in color and each individual snail was a little smaller than a store bought grape. At the top of the slope their was an old animal pen, this year there wasn't any animals in it but the owner of the property said that there had been animals kept in the pen a couple years before the present. About 8 to 10 feet in front of the animal pen were 2 large compost piles. They were both greater than or equal to 10 feet long but less than 20 feet long. The compost piles were greater than or equal to 5 feet wide but less than 10 feet wide. The piles were greater than or equal to 2 feet tall but less than 5 feet tall. In those compost piles there was: corn, grass, dirt, horse manure, chicken manure, dog manure, egg shells, garlic, tomatoes, and apples composted into the piles; that was all that was visible on the surface of the soil.
The soil sample was taken at approximately 4:20 pm on September 16th 2013. According to friendlyforecast.com, the outside temperature was 48.2 degrees Fahrenheit and the sky was cloudy. There was wind blowing from the North at 11.5 miles per hour and gusting up to 21.9 miles per hour. At that hour there was 77% humidity. Earlier that day it rained in Machias Maine, and it rained on our way back to campus from the Salty Dog Farm.
Naming NotesThe phage was named after a loved one. I previously thought of using my loved ones initials, but I decided first name would be best.
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedJan 9, 2014
Sequencing FacilityPittsburgh Bacteriophage Institute
Shotgun Sequencing MethodIllumina Sequencing
Approximate Shotgun Coverage864
Genome length (bp)66507
Character of genome endsCircularly Permuted
GC Content69.0%
Fasta file available?Yes: Download fasta file
Characterization
ClusterB
SubclusterB2
Cluster Life CycleLytic
Other Cluster Members

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AbsoluteMadLad (B1)

ABU (B1)

Adriana (B1)

Aelin (B1)

AltPhacts (B1)

Altwerkus (B1)

Anderson (B1)

Andre (B1)

Antonia (B1)

Apizium (B1)

Ashraf (B1)

Badfish (B1)

Banjo (B1)

Basato (B1)

BatteryCK (B1)

Beaglebox (B1)

Bishoperium (B1)

BlackStallion (B1)

BlueHusk (B1)

Bluephacebaby (B1)

Boehler (B1)

Brilliant (B1)

Buckeye (B1)

Burr (B1)

CamL (B1)

CampRoach (B1)

Cannibal (B1)

Carthage (B1)

Chaelin (B1)

Chah (B1)

Charles1 (B1)

CharlieGBrown (B1)

CheetO (B1)

Cher (B1)

Childish (B1)

Chorkpop (B1)

Chunky (B1)

Chute (B1)

Cobra (B1)

Colbert (B1)

Coletti (B1)

Cornobble (B1)

Cosmolli16 (B1)

Craff (B1)

Crownjwl (B1)

DaddyDaniels (B1)

Daffy (B1)

Daka (B1)

Dati (B1)

DelRivs (B1)

Derpp (B1)

Dice (B1)

Dingo (B1)

Dione (B1)

DirtJuice (B1)

Doddsville (B1)

DoesntMatter (B1)

DonSanchon (B1)

DuchessDung (B1)

Duggie (B1)

Durga (B1)

Emiris (B1)

EmpTee (B1)

Eremos (B1)

Etaye (B1)

Eugenia (B1)

Fang (B1)

FluffyNinja (B1)

Fozzie (B1)

Frankicide (B1)

Freya (B1)

FriarPreacher (B1)

Fringe (B1)

FugateOSU (B1)

Gareth (B1)

GeneCoco (B1)

Giraffe (B1)

Gophee (B1)

Grand2040 (B1)

Gwilliam (B1)

Gyarad (B1)

Haimas (B1)

Haleema (B1)

Hamish (B1)

Hartsy (B1)

Harvey (B1)

Held (B1)

HenryJackson (B1)

Hertubise (B1)

Hetaeria (B1)

HighStump (B1)

Hocus (B1)

Horchata (B1)

HSavage (B1)

ImtiyazSitla (B1)

Inchworm (B1)

Inverness (B1)

Iridoclysis (B1)

IsaacEli (B1)

JacAttac (B1)

JakeO (B1)

JangoPhett (B1)

JDog (B1)

Jillium (B1)

Jiminy (B1)

Jiraiya (B1)

Kahve (B1)

Kailash (B1)

Katniss (B1)

Keitherie (B1)

Kikipoo (B1)

Kimbrough (B1)

KingTut (B1)

KingVeVeVe (B1)

KlimbOn (B1)

Kloppinator (B1)

KLucky39 (B1)

Kwadwo (B1)

Kwksand96 (B1)

Labeouficaum (B1)

LasagnaCat (B1)

Lasso (B1)

LeeLot (B1)

Lego3393 (B1)

Legolas (B1)

LeiMonet (B1)

LemonSlice (B1)

Longacauda (B1)

Lopsy (B1)

LostAndPhound (B1)

LuckyMarjie (B1)

Lulumae (B1)

Lulwa (B1)

Lumine (B1)

Mag7 (B1)

Magic8 (B1)

Mana (B1)

Manad (B1)

Maru (B1)

Maskar (B1)

Matalotodo (B1)

Mcshane (B1)

Mecca (B1)

Megamind20 (B1)

Megatron (B1)

Melc17 (B1)

MelsMeow (B1)

Mesh1 (B1)

MichaelPhcott (B1)

Mikota (B1)

MiniBoss (B1)

MitKao (B1)

Morgushi (B1)

Morty (B1)

Mosaic (B1)

MRabcd (B1)

MrPhizzler (B1)

Mulan (B1)

Murdoc (B1)

Mutante (B1)

Nacho (B1)

Newman (B1)

Nicole21 (B1)

Numberten (B1)

Nyala (B1)

Olak (B1)

Oline (B1)

Olive (B1)

OliverWalter (B1)

Omniscient (B1)

Oosterbaan (B1)

Orfeu (B1)

Orion (B1)

Orwigg (B1)

OSmaximus (B1)

Pacifista (B1)

PG1 (B1)

Phamished (B1)

Phareon (B1)

PhatCats2014 (B1)

PhatLouie (B1)

PhenghisKhan (B1)

Pherdinand (B1)

Phergie (B1)

Phipps (B1)

Phleuron (B1)

PhrankReynolds (B1)

PhrodoBaggins (B1)

Phunky (B1)

Piglet (B1)

PinheadLarry (B1)

Pinkman (B1)

Pipsqueak (B1)

Placalicious (B1)

Plmatters (B1)

Podrick (B1)

Pops (B1)

Potter (B1)

Prickles (B1)

ProfessorX (B1)

Puhltonio (B1)

QueenBeane (B1)

Quisquiliae (B1)

RedMaple (B1)

Ricotta (B1)

Riggan (B1)

Rimu (B1)

Robyn (B1)

Roliet (B1)

Roscoe (B1)

Roy17 (B1)

Samaymay (B1)

SassyCat97 (B1)

Schadenfreude (B1)

Schueller (B1)

Scoot17C (B1)

Scrick (B1)

SDcharge11 (B1)

Selr12 (B1)

Serendipity (B1)

Serpentine (B1)

Sheila (B1)

ShiVal (B1)

Sigman (B1)

SilverBell (B1)

Simielle (B1)

Skippy (B1)

Slatt (B1)

Soile (B1)

Solosis (B1)

Sophia (B1)

Soto (B1)

Spartan300 (B1)

Squid (B1)

Squiggle (B1)

Struggle (B1)

Suffolk (B1)

Surely (B1)

Swiphy (B1)

Swish (B1)

TallGrassMM (B1)

Telesworld (B1)

Thora (B1)

ThreeOh3D2 (B1)

Thunderbird (B1)

Timmi (B1)

TomBombadil (B1)

Tomlarah (B1)

Toni (B1)

Tooj (B1)

True (B1)

Trypo (B1)

TyrionL (B1)

UAch1 (B1)

UncleHowie (B1)

Usavi (B1)

Vaishali24 (B1)

Valjean (B1)

Vaticameos (B1)

Veritas (B1)

Virapocalypse (B1)

Virgeve (B1)

Vista (B1)

Vivaldi (B1)

Vortex (B1)

Wallhey (B1)

Waterdiva (B1)

Weher20 (B1)

Windsor (B1)

Xavier (B1)

Yoshand (B1)

YouGoGlencoco (B1)

Zaider (B1)

Zelda (B1)

Zonia (B1)

Imvubu (B10)

Indlovu (B11)

LilMcDreamy (B12)

BirdsNest (B13)

Hashim76 (B13)

Zenteno07 (B13)

Allegro (B2)

Arbiter (B2)

Ares (B2)

Bananafish (B2)

Blocker23 (B2)

Boyle (B2)

Brownie5 (B2)

Calamitous (B2)

Coffee (B2)

Eaglehorse (B2)

Faze9 (B2)

FrenchFry (B2)

Glass (B2)

Godines (B2)

Hedgerow (B2)

Holeinone (B2)

ItsyBitsy1 (B2)

Kaleb (B2)

Kheth (B2)

Lars (B2)

Laurie (B2)

Lephleur (B2)

LizLemon (B2)

MasterPo (B2)

Opia (B2)

Phantasmagoria (B2)

Qyrzula (B2)

Rhinoforte (B2)

Rosebush (B2)

Sabella (B2)

TA17A (B2)

Tinciduntsolum (B2)

Tres (B2)

Vic9 (B2)

West99 (B2)

Abinghost (B3)

Akoma (B3)

Athena (B3)

Audrey (B3)

Baloo (B3)

Ben11 (B3)

Bernardo (B3)

Briakila (B3)

Casbah (B3)

ChaChing (B3)

Chandler (B3)

Compostia (B3)

Corofin (B3)

Daisy (B3)

Deenasa (B3)

Devonte (B3)

Gadjet (B3)

Gervas (B3)

GlenHope (B3)

Halfpint (B3)

HarveySr (B3)

Heathcliff (B3)

Jackstina (B3)

Kamiyu (B3)

Kronus (B3)

LestyG (B3)

Marley1013 (B3)

MmasiCarm (B3)

Mortcellus (B3)

Morty007 (B3)

Neos5 (B3)

Nozo (B3)

Obutu (B3)

OrangeOswald (B3)

Phaedrus (B3)

Phayeta (B3)

Philly (B3)

Phlyer (B3)

Pipefish (B3)

RagingRooster (B3)

Rita1961 (B3)

RomaT (B3)

SlippinJimmy (B3)

SynergyX (B3)

Tydolla (B3)

Yahalom (B3)

Yinz (B3)

Ahwei (B4)

AlanGrant (B4)

Antihero (B4)

Apex (B4)

Austelle (B4)

BobbyK (B4)

BrownCNA (B4)

ChrisnMich (B4)

Cooper (B4)

Epah (B4)

Fanaw (B4)

Fortunato (B4)

Frederick (B4)

GinPorsche (B4)

Hally898 (B4)

Hangman (B4)

Heath (B4)

Hydro (B4)

JaguarMi (B4)

JAMaL (B4)

Lambano (B4)

Lolalove (B4)

Magpie (B4)

Mithril (B4)

Mudslide (B4)

Nanao (B4)

Nigel (B4)

Poster (B4)

Prince (B4)

QueenPie (B4)

RawrgerThat (B4)

Stinger (B4)

Vincenzo (B4)

VioletZ (B4)

Waleliano (B4)

Xincheng (B4)

Zemanar (B4)

Acadian (B5)

Baee (B5)

DigitDog (B5)

Donny (B5)

EastView9101 (B5)

KarasumaRum (B5)

Mysterious (B5)

Nangang (B5)

Nehemiah (B5)

Phelemich (B5)

Reprobate (B5)

Rich (B5)

Serendipitous (B5)

Shenyu (B5)

SirJeffery (B5)

StinkyVeggy (B5)

Suigeneris (B5)

39HC (B6)

40BC (B6)

Hosp (B6)

Jolie1 (B6)

KayaCho (B6)

Saguaro (B7)

Thonko (B8)

CRB2 (B9)

Funsized (B9)

Knocker (B9)

PenguinLover67 (B9)

Quesadilla (B9)

Rinkes (B9)

Annotating InstitutionUnknown or unassigned
Annotation StatusIn GenBank
Plaque Notes0.5-2.0 mm plaques at 24 hours.
MorphotypeSiphoviridae
Number of Genes90
Number of tRNAs0
Number of tmRNAs0
Has been Phamerated?Yes
Gene List

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Laurie_1

Laurie_2
(queuine tRNA-ribosyltransferase)

Laurie_3
(Pre Qo pathway QueC-like protein)

Laurie_4

Laurie_5
(QueE-like queosine biosynthesis protein)

Laurie_6
(GTP cyclohydrolase I)

Laurie_7
(terminase)

Laurie_8

Laurie_9
(RuvC-like resolvase)

Laurie_10

Laurie_11

Laurie_12
(portal protein)

Laurie_13
(MuF-like minor capsid protein)

Laurie_14

Laurie_15
(major capsid hexamer protein)

Laurie_16
(major capsid pentamer protein)

Laurie_17

Laurie_18

Laurie_19

Laurie_20

Laurie_21
(major tail protein)

Laurie_22

Laurie_23

Laurie_24
(head-to-tail adaptor)

Laurie_25

Laurie_26

Laurie_27
(tail assembly chaperone)

Laurie_28

Laurie_29
(tape measure protein)

Laurie_30
(minor tail protein)

Laurie_31
(minor tail protein)

Laurie_32
(minor tail protein)

Laurie_33
(minor tail protein)

Laurie_34

Laurie_35

Laurie_36

Laurie_37

Laurie_38

Laurie_39

Laurie_40

Laurie_41

Laurie_42

Laurie_43
(helix-turn-helix DNA binding domain protein)

Laurie_44

Laurie_45

Laurie_46
(lysin A)

Laurie_47
(holin)

Laurie_48

Laurie_49

Laurie_50
(DNA helicase)

Laurie_51

Laurie_52

Laurie_53

Laurie_54
(DNA primase/helicase)

Laurie_55

Laurie_56

Laurie_57
(DNA polymerase I)

Laurie_58

Laurie_59

Laurie_60

Laurie_61

Laurie_62

Laurie_63

Laurie_64

Laurie_65

Laurie_66
(DNA binding protein)

Laurie_67

Laurie_68

Laurie_69

Laurie_70

Laurie_71
(HNH endonuclease)

Laurie_72

Laurie_73

Laurie_74

Laurie_75

Laurie_76

Laurie_77

Laurie_78

Laurie_79

Laurie_80

Laurie_81

Laurie_82

Laurie_83

Laurie_84

Laurie_85

Laurie_86

Laurie_87

Laurie_88

Laurie_89

Laurie_90

Submitted Annotation in DNA Master FormatDownload
Submitted Minimal DNA Master FileDownload
Author ListDownload
Annotation Cover SheetDownload
Publication Info
Uploaded to GenBank?Yes
GenBank AccessionKX443696
Refseq NumberNone yet
Published in PaperPope, Bowman, Russell, et al 2015, eLIFE: Whole genome comparison of a large collection of mycobacteriophages reveals a continuum of phage genetic diversity
Archiving Info
Archiving status Archived
Pitt Freezer Box# 5
Pitt Freezer Box Grid# G11
Available Files
Plaque PictureDownload
EM PictureDownload
Final DNAMaster FileDownload
GenBank File for PhameratorDownload