The Actinobacteriophage Database

Mycobacterium phage Mudslide

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Detailed Information for Phage Mudslide
Discovery Information
Isolation Host	Mycobacterium smegmatis mc²155
Found By	Gabrielle Cruz
Year Found	2022
Location Found	Collegeville, PA United States of America
Finding Institution	Saint Joseph's University
Program	Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?	Yes
Isolation Temperature	37°C
GPS Coordinates	40.180902 N, 75.482382 W Map
Discovery Notes	Phage was collected on January 17th, 2022 at 3:40pm at Anderson Farm Park, Collegeville PA, 19426, near small stream on the building side. Soil condition: damp, hard clay, brittle, frozen Soil depth: 11.37 cm Ambiente temperature: 1.67 C Humidity: 67% Weather conditions: flurries, cloudy, windy
Naming Notes	When approaching the site where this phage was discovered, I slipped in mud. Therefore, this phage was named "Mudslide."
Sequencing Information
Sequencing Complete?	Yes
Date Sequencing Completed	Jul 12, 2022
Sequencing Facility	Pittsburgh Bacteriophage Institute
Shotgun Sequencing Method	Illumina
Sequencer Used	Illumina MiSeq
Read Type	Single-end reads
Read Length	150 bp
Approximate Shotgun Coverage	691
Genome length (bp)	71342
Character of genome ends	Circularly Permuted
GC Content	68.8%
Fasta file available?	Yes: Download fasta file
Characterization
Cluster	B
Subcluster	B4
Cluster Life Cycle	Lytic
Other Cluster Members	Click to View AbsoluteMadLad (B1) ABU (B1) Adriana (B1) Aelin (B1) AltPhacts (B1) Altwerkus (B1) Anderson (B1) Andre (B1) AngelCake (B1) Antonia (B1) Apizium (B1) Ashraf (B1) Badfish (B1) Banjo (B1) Basato (B1) BatteryCK (B1) Beaglebox (B1) Bishoperium (B1) BlackStallion (B1) BlueHusk (B1) Bluephacebaby (B1) Boehler (B1) Brilliant (B1) Buckeye (B1) Burr (B1) CamL (B1) CampRoach (B1) Cannibal (B1) Carthage (B1) Caspiboi (B1) Chaelin (B1) Chah (B1) Charles1 (B1) CharlieGBrown (B1) CheetO (B1) Cher (B1) Childish (B1) Chipette (B1) Chorkpop (B1) Chunky (B1) Chute (B1) Cobra (B1) Colbert (B1) Coletti (B1) Cornbread (B1) Cornobble (B1) Cosmolli16 (B1) Craff (B1) Crownjwl (B1) DaddyDaniels (B1) Daffy (B1) Daka (B1) Dati (B1) DelRivs (B1) Derpp (B1) Dice (B1) Dingo (B1) Dione (B1) DirtJuice (B1) Doddsville (B1) DoesntMatter (B1) DonSanchon (B1) DuchessDung (B1) Duggie (B1) Durga (B1) ElvisPhasley (B1) Emiris (B1) EmpTee (B1) Eremos (B1) Etaye (B1) Eugenia (B1) Fang (B1) FluffyNinja (B1) Fozzie (B1) Frankicide (B1) FremontTroll (B1) Freya (B1) FriarPreacher (B1) Fringe (B1) FugateOSU (B1) Gareth (B1) GeneCoco (B1) Giraffe (B1) Gophee (B1) Grand2040 (B1) Gwennie1 (B1) Gwilliam (B1) Gyarad (B1) Haimas (B1) Haleema (B1) Hamish (B1) Hartsy (B1) Harvey (B1) Held (B1) HenryJackson (B1) Hertubise (B1) Hetaeria (B1) HighStump (B1) Hocus (B1) Horchata (B1) HSavage (B1) ImtiyazSitla (B1) Inchworm (B1) Inverness (B1) Iridessa (B1) Iridoclysis (B1) IsaacEli (B1) JacAttac (B1) JakeO (B1) JangoPhett (B1) JDog (B1) Jesabah (B1) Jillium (B1) Jiminy (B1) Jiraiya (B1) Kahve (B1) Kailash (B1) Katniss (B1) Keitherie (B1) Khan1 (B1) Kikipoo (B1) Kimbrough (B1) KingTut (B1) KingVeVeVe (B1) KlimbOn (B1) Kloppinator (B1) KLucky39 (B1) Kwadwo (B1) Kwksand96 (B1) Labeouficaum (B1) LasagnaCat (B1) Lasso (B1) LeeLot (B1) Lego3393 (B1) Legolas (B1) Lehmann (B1) LeiMonet (B1) LemonSlice (B1) Longacauda (B1) Lopsy (B1) LostAndPhound (B1) LuckyMarjie (B1) Lulumae (B1) Lulwa (B1) Lumine (B1) Mag7 (B1) Magic8 (B1) Mana (B1) Manad (B1) Maru (B1) Maskar (B1) Matalotodo (B1) Mcshane (B1) Mecca (B1) Megamind20 (B1) Megatron (B1) Melc17 (B1) MelsMeow (B1) Mesh1 (B1) Mesmerelda (B1) MichaelPhcott (B1) Mikota (B1) MiniBoss (B1) MitKao (B1) Morgushi (B1) Morty (B1) Mosaic (B1) MRabcd (B1) MrPhizzler (B1) Mulan (B1) Murdoc (B1) Mutante (B1) Nacho (B1) Nathan (B1) Newman (B1) Nicole21 (B1) Numberten (B1) Nyala (B1) Olak (B1) Oline (B1) Olive (B1) OliverWalter (B1) Omniscient (B1) Oosterbaan (B1) Orfeu (B1) Orion (B1) Orwigg (B1) OSmaximus (B1) Pacifista (B1) PG1 (B1) Phamished (B1) Phareon (B1) PhatCats2014 (B1) PhatLouie (B1) PhenghisKhan (B1) Pherdinand (B1) Phergie (B1) Phipps (B1) Phleuron (B1) PhrankReynolds (B1) PhrodoBaggins (B1) Phunky (B1) Piglet (B1) PinheadLarry (B1) Pinkman (B1) Pipsqueak (B1) Placalicious (B1) Plata (B1) Plmatters (B1) Podrick (B1) Pops (B1) Potter (B1) Prickles (B1) ProfessorX (B1) Puhltonio (B1) QueenBeane (B1) Quisquiliae (B1) RedMaple (B1) Ricotta (B1) Riggan (B1) Rimu (B1) Riverton (B1) Robyn (B1) Roliet (B1) Roscoe (B1) Roy17 (B1) Samaymay (B1) SassyCat97 (B1) Schadenfreude (B1) Schueller (B1) Scoot17C (B1) Scrick (B1) SDcharge11 (B1) Selr12 (B1) Serendipity (B1) Serpentine (B1) Sheila (B1) ShiVal (B1) Sigman (B1) SilverBell (B1) Simielle (B1) SirBeauregard (B1) Skippy (B1) Slatt (B1) Soile (B1) Solosis (B1) Sophia (B1) Soto (B1) Spartan300 (B1) Squid (B1) Squiggle (B1) Struggle (B1) Suffolk (B1) Surely (B1) Swiphy (B1) Swish (B1) TallGrassMM (B1) Telesworld (B1) Thora (B1) ThreeOh3D2 (B1) Thunderbird (B1) Timmi (B1) TomBombadil (B1) Tomlarah (B1) Toni (B1) TooFast2Furius (B1) Tooj (B1) Triad (B1) True (B1) Trypo (B1) TyrionL (B1) UAch1 (B1) UncleHowie (B1) Usavi (B1) Vaishali24 (B1) Valjean (B1) Vaticameos (B1) Veritas (B1) Virapocalypse (B1) Virgeve (B1) Vista (B1) Vivaldi (B1) Vortex (B1) Wallhey (B1) Waterdiva (B1) Weher20 (B1) Windsor (B1) Xavier (B1) Yoshand (B1) YouGoGlencoco (B1) Zaider (B1) Zelda (B1) Zonia (B1) Imvubu (B10) Indlovu (B11) LilMcDreamy (B12) BirdsNest (B13) Hashim76 (B13) LittleMinkey (B13) Zenteno07 (B13) Allegro (B2) Arbiter (B2) Ares (B2) Bananafish (B2) Blocker23 (B2) Boyle (B2) Brownie5 (B2) Calamitous (B2) Coffee (B2) Eaglehorse (B2) Faze9 (B2) FrenchFry (B2) Glass (B2) Godines (B2) Hedgerow (B2) Holeinone (B2) ItsyBitsy1 (B2) Kaleb (B2) Kheth (B2) Kolhan (B2) Lars (B2) Laurie (B2) Lephleur (B2) LilSmirk (B2) LizLemon (B2) MasterPo (B2) Opia (B2) Phantasmagoria (B2) PrinceCharming (B2) Qyrzula (B2) Rhinoforte (B2) Rosebush (B2) Sabella (B2) TA17A (B2) Tinciduntsolum (B2) Tres (B2) Vic9 (B2) West99 (B2) Abinghost (B3) Akoma (B3) Athena (B3) Audrey (B3) Baloo (B3) Ben11 (B3) Bernardo (B3) Briakila (B3) Casbah (B3) ChaChing (B3) Chalmers (B3) Chandler (B3) Chentzyk (B3) Compostia (B3) Corofin (B3) Daisy (B3) Deenasa (B3) Devonte (B3) Gadjet (B3) Gervas (B3) GlenHope (B3) Glubnar (B3) Halfpint (B3) HarveySr (B3) Heathcliff (B3) Jackstina (B3) Kamiyu (B3) Kronus (B3) LestyG (B3) Marley1013 (B3) Marlia (B3) MmasiCarm (B3) Mortcellus (B3) Morty007 (B3) Neos5 (B3) Nozo (B3) Obutu (B3) Olivio (B3) OrangeOswald (B3) Phaedrus (B3) Phayeta (B3) Philly (B3) Phlyer (B3) Pipefish (B3) RagingRooster (B3) Rita1961 (B3) RomaT (B3) Shenandoah (B3) SlippinJimmy (B3) SwainyDoc (B3) SynergyX (B3) TinyTerror (B3) Tydolla (B3) Yahalom (B3) Yinz (B3) Ahwei (B4) AlanGrant (B4) Antihero (B4) Apex (B4) Austelle (B4) BobbyK (B4) BrownCNA (B4) ChrisnMich (B4) Cooper (B4) Epah (B4) Fanaw (B4) Fortunato (B4) Frederick (B4) GinPorsche (B4) Hally898 (B4) Hangman (B4) Heath (B4) Hydro (B4) JaguarMi (B4) JAMaL (B4) Lambano (B4) Lolalove (B4) Magpie (B4) Mithril (B4) Mudslide (B4) Nanao (B4) Nigel (B4) Poster (B4) Prince (B4) QueenPie (B4) RawrgerThat (B4) Stinger (B4) Vincenzo (B4) VioletZ (B4) Waleliano (B4) Xincheng (B4) Zemanar (B4) Acadian (B5) Baee (B5) DigitDog (B5) Donny (B5) EastView9101 (B5) KarasumaRum (B5) Mysterious (B5) Nangang (B5) Nehemiah (B5) Phelemich (B5) Reprobate (B5) Rich (B5) Rita130 (B5) Serendipitous (B5) Shenyu (B5) SirJeffery (B5) StinkyVeggy (B5) Suigeneris (B5) 39HC (B6) 40BC (B6) ForDig (B6) Hosp (B6) Jolie1 (B6) KayaCho (B6) Saguaro (B7) Thonko (B8) CRB2 (B9) Funsized (B9) Knocker (B9) PenguinLover67 (B9) Quesadilla (B9) Rinkes (B9)
Annotating Institution	Saint Joseph's University
Annotation Status	In GenBank
Plaque Notes	Clear, 1 mm plaques Incubation length: 48 hours
Morphotype	Siphoviridae
Number of Genes	98
Number of tRNAs	0
Number of tmRNAs	0
Has been Phamerated?	Yes
Gene List	Click to View Mudslide_1 (ParB-like nuclease domain protein) Mudslide_2 Mudslide_3 Mudslide_4 (adenylate kinase) Mudslide_5 (terminase) Mudslide_6 Mudslide_7 (RuvC-like resolvase) Mudslide_8 Mudslide_9 (portal protein) Mudslide_10 (capsid maturation protease) Mudslide_11 Mudslide_12 (major capsid hexamer protein) Mudslide_13 (major capsid pentamer protein) Mudslide_14 Mudslide_15 (holin) Mudslide_16 Mudslide_17 Mudslide_18 (major tail protein) Mudslide_19 Mudslide_20 (head-to-tail adaptor) Mudslide_21 Mudslide_22 Mudslide_23 (tail assembly chaperone) Mudslide_24 Mudslide_25 (tape measure protein) Mudslide_26 (minor tail protein) Mudslide_27 (minor tail protein) Mudslide_28 (minor tail protein) Mudslide_29 (minor tail protein) Mudslide_30 (minor tail protein) Mudslide_31 Mudslide_32 Mudslide_33 Mudslide_34 Mudslide_35 Mudslide_36 Mudslide_37 Mudslide_38 Mudslide_39 Mudslide_40 (helix-turn-helix DNA binding domain protein) Mudslide_41 (helix-turn-helix DNA binding domain protein) Mudslide_42 Mudslide_43 (lysin A) Mudslide_44 (lysin B) Mudslide_45 Mudslide_46 Mudslide_47 (DNA helicase) Mudslide_48 Mudslide_49 Mudslide_50 Mudslide_51 (DNA primase/polymerase/helicase) Mudslide_52 Mudslide_53 (DNA polymerase I) Mudslide_54 Mudslide_55 Mudslide_56 Mudslide_57 Mudslide_58 Mudslide_59 Mudslide_60 Mudslide_61 (ribbon-helix-helix DNA binding domain protein) Mudslide_62 Mudslide_63 Mudslide_64 Mudslide_65 Mudslide_66 Mudslide_67 Mudslide_68 Mudslide_69 Mudslide_70 Mudslide_71 Mudslide_72 Mudslide_73 Mudslide_74 Mudslide_75 (HNH endonuclease) Mudslide_76 Mudslide_77 Mudslide_78 Mudslide_79 Mudslide_80 Mudslide_81 Mudslide_82 Mudslide_83 Mudslide_84 Mudslide_85 Mudslide_86 Mudslide_87 Mudslide_88 Mudslide_89 Mudslide_90 Mudslide_91 Mudslide_92 Mudslide_93 Mudslide_94 Mudslide_95 Mudslide_96 Mudslide_97 Mudslide_98
Submitted Annotation in DNA Master Format	Download
Submitted Minimal DNA Master File	Download
Author List	Download
Annotation Cover Sheet	Download
Publication Info
Uploaded to GenBank?	Yes
GenBank Accession	OR475251
Refseq Number	None yet
Archiving Info
Archiving status	Archived
Pitt Freezer Box#	146
Pitt Freezer Box Grid#	B3
Available Files
Plaque Picture	Download
Restriction Digest Picture	Download
EM Picture	Download
GenBank File for Phamerator	Download

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