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Mycobacterium phage BaboJay
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Detailed Information for Phage BaboJay
Discovery Information
Isolation HostMycobacterium smegmatis mc²155
Found ByRethabile leqhaoe
Year Found2014
Location FoundDurban, Kwa-Zulu Natal, South-Africa
Finding InstitutionUniversity of Kwazulu-Natal
ProgramMycobacterial Genetics Course, Durban, South Africa
From enriched soil sample?No
Isolation TemperatureNot entered
GPS Coordinates29.81802 S, 30.94418 E Map
Discovery NotesFound in a garden on the university of Kwa-zulu Natal, in a garden of flower, to obtain the sample, I dug the superficial soil layer, the soil was therefore dry. The garden has a tree nearby.
the sample has been purified three times
Naming NotesThe phage is named for my little sister, Nts'ebo Josette. we nicknamed her Babo, hence the first part of the name, the second part is the initial letter of her middle name(Josette), subsequently giving BaboJay... I named the phage after her because of the halo plaque morphologies, it deserved to be named after an angel...
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedOct 1, 2015
Sequencing FacilityPittsburgh Bacteriophage Institute
Approximate Shotgun Coverage1770
Genome length (bp)76377
Character of genome ends3' Sticky Overhang
Overhang Length9 bases
Overhang SequenceCGCTTGTCA
GC Content62.9%
Fasta file available?Yes: Download fasta file
Characterization
ClusterE
Subcluster--
Cluster Life CycleTemperate
Other Cluster Members
Annotation StatusIn GenBank
Plaque Notesthe plaque had a clear zone, surrounded by a very turbid circular zone, finished off by a lightly turbid center, giving off a halo appearance.
MorphotypeSiphoviridae
Has been Phamerated?Yes
Gene List

Click to View

BaboJay_1

BaboJay_2

BaboJay_3

BaboJay_4

BaboJay_5

BaboJay_6
(terminase, small subunit)

BaboJay_7

BaboJay_8
(terminase, large subunit)

BaboJay_9

BaboJay_10
(portal protein)

BaboJay_11
(capsid maturation protease)

BaboJay_12
(major capsid protein)

BaboJay_13

BaboJay_14
(head-to-tail adaptor)

BaboJay_15
(head-to-tail stopper)

BaboJay_16

BaboJay_17
(tail terminator)

BaboJay_18
(major tail protein)

BaboJay_19
(tail assembly chaperone)

BaboJay_20
(tail assembly chaperone)

BaboJay_21
(tape measure protein)

BaboJay_22

BaboJay_23

BaboJay_24
(major tail)

BaboJay_25
(major tail)

BaboJay_26
(major tail)

BaboJay_27

BaboJay_28

BaboJay_29
(helix-turn-helix DNA binding protein)

BaboJay_30

BaboJay_31
(lysin A)

BaboJay_32
(holin)

BaboJay_33

BaboJay_34
(lysin B)

BaboJay_35

BaboJay_36
(HNH endonuclease)

BaboJay_37
(NrdH-like glutaredoxin)

BaboJay_38

BaboJay_39

BaboJay_40

BaboJay_41

BaboJay_42

BaboJay_43

BaboJay_44

BaboJay_45

BaboJay_46

BaboJay_47

BaboJay_48
(integrase (Y-Int))

BaboJay_49

BaboJay_50

BaboJay_51
(helix-turn-helix DNA binding protein)

BaboJay_52

BaboJay_53

BaboJay_54

BaboJay_55

BaboJay_56

BaboJay_57

BaboJay_58

BaboJay_59

BaboJay_60

BaboJay_61

BaboJay_62

BaboJay_63

BaboJay_64
(exonuclease)

BaboJay_65

BaboJay_66

BaboJay_67

BaboJay_68

BaboJay_69

BaboJay_70

BaboJay_71

BaboJay_72

BaboJay_73
(DNA binding protein)

BaboJay_74
(HNH endonuclease)

BaboJay_75

BaboJay_76

BaboJay_77

BaboJay_78
(dsDNA helicase)

BaboJay_79

BaboJay_80
(HNH endonuclease)

BaboJay_81

BaboJay_82

BaboJay_83

BaboJay_84
(Holliday junction resolvase)

BaboJay_85

BaboJay_86
(polynucleotide kinase)

BaboJay_87

BaboJay_88

BaboJay_89
(RNA ligase)

BaboJay_90
(HNH endonuclease)

BaboJay_91
(RNA ligase)

BaboJay_92
(helix-turn-helix DNA binding protein)

BaboJay_93

BaboJay_94

BaboJay_95

BaboJay_96

BaboJay_97

BaboJay_98

BaboJay_99
(methyltransferase)

BaboJay_100
(ssDNA binding protein)

BaboJay_101
(ClpP-like protease)

BaboJay_102

BaboJay_103

BaboJay_104
(helix-turn-helix DNA binding protein)

BaboJay_105

BaboJay_108

BaboJay_109

BaboJay_110
(HNH endonuclease)

BaboJay_111
(deoxycytidylate deaminase)

BaboJay_112

BaboJay_113
(DNA polymerase I)

BaboJay_114

BaboJay_115
(RecA-like recombinase)

BaboJay_116

BaboJay_117

BaboJay_118

BaboJay_119

BaboJay_120

BaboJay_121

BaboJay_122

BaboJay_123

BaboJay_124

BaboJay_125

BaboJay_126

BaboJay_127
(DNA binding protein)

BaboJay_128

BaboJay_129

BaboJay_130

BaboJay_131

BaboJay_132

BaboJay_133

BaboJay_134

BaboJay_135

BaboJay_136

BaboJay_137

BaboJay_138

BaboJay_139

BaboJay_140

BaboJay_141

BaboJay_142

BaboJay_143

BaboJay_144

BaboJay_145

BaboJay_146
(HNH endonuclease)

Submitted Minimal DNA Master FileDownload
Publication Info
Uploaded to GenBank?Yes
GenBank AccessionMK016491
Refseq NumberNone yet
Archiving Info
Archiving status Archived
Pitt Freezer Box# 7
Pitt Freezer Box Grid# C8
Available Files
Plaque PictureDownload
EM PictureDownload
Final DNAMaster FileDownload
GenBank File for PhameratorDownload