Mycobacterium phage Pat3
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Detailed Information for Phage Pat3
Discovery Information
Isolation HostMycobacterium smegmatis mc²155
Found ByJames Kuan Broughton
Year Found2015
Location FoundDurban, South Africa
Finding InstitutionUniversity of Kwazulu-Natal
ProgramMycobacterial Genetics Course, Durban, South Africa
From enriched soil sample?Yes
Isolation Temperature37°C
GPS Coordinates29.8607 S, 30.980314 E Map
Discovery NotesSoil from a flower pot in my garden, it is watered regularly and receives a lot of sunlight; half red sand taken from surrounding garden, half compost; relatively dry; taken from surface layer.
Naming NotesName is my Gran's nickname. She taught me everything she knew about gardening when I was young, and since the soil is from a flower pot, I decided to name the phage after her.
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedOct 6, 2015
Sequencing FacilityPittsburgh Bacteriophage Institute
Shotgun Sequencing MethodIllumina Sequencing
Approximate Shotgun Coverage1534
Genome length (bp)75675
Character of genome ends3' Sticky Overhang
Overhang Length9 bases
Overhang SequenceCGCTTGTCA
GC Content62.9%
Fasta file available?Yes: Download fasta file
Cluster Life CycleTemperate
Other Cluster Members
Annotation StatusBeing Annotated (Expected completion by 9/1/2018)
Plaque NotesLarge plaques (~4.5-5mm diameter) develop after 2 days overnight incubation at 37 deg. celsius with a uniform circular morphology, and distinct halos. Halos only form after 2 days of incubation. After 1 overnight incubation at 37 deg. celsius, plaques are small ~1-2mm diameter and halos are absent. Plaques did not form after 3 days incubation at room temperature (at the time ~20 deg. celsius).
Has been Phamerated?Yes
Gene List
Publication Info
Uploaded to GenBank?No
GenBank AccessionNone yet
Refseq NumberNone yet
Archiving Info
Archiving status Archived
SEA Lysate Titer 2x10^10 PFU/ml
Date of SEA Lysate Titering Aug 7, 2015
Available Files
Plaque PictureDownload
EM PictureDownload