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Mycobacterium phage Pat3
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Detailed Information for Phage Pat3
Discovery Information
Isolation HostMycobacterium smegmatis mc²155
Found ByJames Kuan Broughton
Year Found2015
Location FoundDurban, South Africa
Finding InstitutionUniversity of Kwazulu-Natal
ProgramMycobacterial Genetics Course, Durban, South Africa
From enriched soil sample?Yes
Isolation Temperature37°C
GPS Coordinates29.8607 S, 30.980314 E Map
Discovery NotesSoil from a flower pot in my garden, it is watered regularly and receives a lot of sunlight; half red sand taken from surrounding garden, half compost; relatively dry; taken from surface layer.
Naming NotesName is my Gran's nickname. She taught me everything she knew about gardening when I was young, and since the soil is from a flower pot, I decided to name the phage after her.
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedOct 6, 2015
Sequencing FacilityPittsburgh Bacteriophage Institute
Shotgun Sequencing MethodIllumina Sequencing
Approximate Shotgun Coverage1534
Genome length (bp)75675
Character of genome ends3' Sticky Overhang
Overhang Length9 bases
Overhang SequenceCGCTTGTCA
GC Content62.9%
Fasta file available?Yes: Download fasta file
Characterization
ClusterE
Subcluster--
Cluster Life CycleTemperate
Lysogeny NotesImmune to phage CJWI (cluster E)
Other Cluster Members
Annotation StatusIn GenBank
Plaque NotesLarge plaques (~4.5-5mm diameter) develop after 2 days overnight incubation at 37 deg. celsius with a uniform circular morphology, and distinct halos. Halos only form after 2 days of incubation. After 1 overnight incubation at 37 deg. celsius, plaques are small ~1-2mm diameter and halos are absent. Plaques did not form after 3 days incubation at room temperature (at the time ~20 deg. celsius).
MorphotypeSiphoviridae
Has been Phamerated?Yes
Gene List

Click to View

Pat3_1

Pat3_2

Pat3_3

Pat3_4

Pat3_5

Pat3_6
(terminase, small subunit)

Pat3_7

Pat3_8
(terminase, large subunit)

Pat3_9

Pat3_10
(portal protein)

Pat3_11
(capsid maturation protease)

Pat3_12
(major capsid protein)

Pat3_13

Pat3_14
(head-to-tail adaptor)

Pat3_15
(head-to-tail stopper)

Pat3_16

Pat3_17
(tail terminator)

Pat3_18
(major tail)

Pat3_19
(tail assembly chaperone)

Pat3_20
(tail assembly chaperone)

Pat3_21
(tape measure protein)

Pat3_22
(major tail)

Pat3_23
(major tail)

Pat3_24
(major tail)

Pat3_25
(major tail)

Pat3_26
(major tail)

Pat3_27

Pat3_28

Pat3_29
(helix-turn-helix DNA binding protein)

Pat3_30

Pat3_31
(lysin A)

Pat3_32
(holin)

Pat3_33

Pat3_34
(lysin B)

Pat3_35

Pat3_36

Pat3_37

Pat3_38

Pat3_39

Pat3_40

Pat3_41

Pat3_42

Pat3_43

Pat3_44

Pat3_45

Pat3_46

Pat3_47

Pat3_48

Pat3_49

Pat3_50
(integrase, (Y-Int))

Pat3_51

Pat3_52

Pat3_53
(helix-turn-helix DNA binding protein)

Pat3_54

Pat3_55

Pat3_56

Pat3_57

Pat3_58

Pat3_59

Pat3_60

Pat3_61

Pat3_62

Pat3_63

Pat3_64

Pat3_65
(exonuclease)

Pat3_66

Pat3_67

Pat3_68

Pat3_69

Pat3_70

Pat3_71

Pat3_72

Pat3_73

Pat3_74
(DNa binding protein)

Pat3_75
(HNH endonuclease)

Pat3_76

Pat3_77

Pat3_78

Pat3_79
(dsDNA helicase)

Pat3_80

Pat3_81

Pat3_82

Pat3_83

Pat3_84
(Holliday junction resolvase)

Pat3_85

Pat3_86
(polynucleotide kinase)

Pat3_87

Pat3_88
(RNA ligase)

Pat3_89
(helix-turn-helix DNA binding protein)

Pat3_90

Pat3_91

Pat3_92

Pat3_93

Pat3_94

Pat3_95

Pat3_96
(DNA methylase)

Pat3_97
(ssDNA binding protein)

Pat3_98
(ClpP-like protease)

Pat3_99

Pat3_100

Pat3_101

Pat3_102
(helix-turn-helix DNA binding protein)

Pat3_103
(HNH endonuclease)

Pat3_106

Pat3_107

Pat3_108
(deoxycytidylate deaminase)

Pat3_109

Pat3_110

Pat3_111
(DNA polymerase I)

Pat3_112

Pat3_113

Pat3_114

Pat3_115
(RecA-like recombinase)

Pat3_116

Pat3_117

Pat3_118

Pat3_119

Pat3_120

Pat3_121

Pat3_122

Pat3_123

Pat3_124

Pat3_125

Pat3_126

Pat3_127
(DNA binding protein)

Pat3_128

Pat3_129

Pat3_130

Pat3_131

Pat3_132

Pat3_133

Pat3_134

Pat3_135

Pat3_136

Pat3_137

Pat3_138

Pat3_139

Pat3_140

Pat3_141

Pat3_142

Pat3_143

Pat3_144

Pat3_145

Pat3_146

Pat3_147
(HNH endonuclease)

Submitted Minimal DNA Master FileDownload
Publication Info
Uploaded to GenBank?Yes
GenBank AccessionMK016502
Refseq NumberNone yet
Archiving Info
Archiving status Archived
SEA Lysate Titer 2x10^10 PFU/ml
Date of SEA Lysate Titering Aug 7, 2015
Available Files
Plaque PictureDownload
EM PictureDownload
Final DNAMaster FileDownload
GenBank File for PhameratorDownload